Department of Immunology, Genetics and Immunology, Uppsala University, S-751 85 Uppsala, Sweden.
Department of Immunology, Genetics and Immunology, Uppsala University, S-751 85 Uppsala, Sweden.
Virology. 2014 May;456-457:329-41. doi: 10.1016/j.virol.2014.04.006. Epub 2014 Apr 25.
Adenovirus type 2 RNA splicing events were quantitatively mapped by using deep cDNA sequencing. The majority of the previously identified splice sites were detected. The lack of complete consistency between the present and previous results is because of some sites which were incorrectly mapped in previous studies, such as the splice sites for pVII, pVIII and E3-11.6K. Several previously predicted splice sites such as that for E3-14.5K and E4ORF3/4 were not detected. In addition, several new splice sites were identified. The novel RNAs may code for hitherto undetected proteins or alternatively spliced mRNAs for known proteins. The open reading frames downstream of two novel splice sites, located in the major late transcription unit region, were shown to be highly conserved. Another interesting possibility is that some of them are non-coding RNAs. Finally, the adenovirus mRNA polyadenylation sites were accurately mapped and in some cases shown to be heterogeneous.
采用深度 cDNA 测序技术对腺病毒 2 型 RNA 剪接事件进行了定量映射。大多数先前鉴定的剪接位点都被检测到。目前的结果与以前的结果不完全一致，原因是以前的研究中有些位点映射不正确，例如 pVII、pVIII 和 E3-11.6K 的剪接位点。几个先前预测的剪接位点，如 E3-14.5K 和 E4ORF3/4，没有被检测到。此外，还鉴定了几个新的剪接位点。这些新的 RNA 可能编码以前未检测到的蛋白质，或者为已知蛋白质编码可变剪接的 mRNA。位于主要晚期转录单元区域的两个新剪接位点下游的开放阅读框高度保守。另一个有趣的可能性是，其中一些是非编码 RNA。最后，准确地映射了腺病毒 mRNA 的多聚腺苷酸化位点，并且在某些情况下显示出异质性。
