Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA 02115, United States; Division of Genetics, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, United States; Division of Cardiovascular Medicine, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, United States.
Semin Cell Dev Biol. 2013 Aug-Sep;24(8-9):661-7. doi: 10.1016/j.semcdb.2013.05.001. Epub 2013 May 7.
Multi-isotope imaging mass spectrometry (MIMS) is the quantitative imaging of stable isotope labels in cells with a new type of secondary ion mass spectrometer (NanoSIMS). The power of the methodology is attributable to (i) the immense advantage of using non-toxic stable isotope labels, (ii) high resolution imaging that approaches the resolution of usual transmission electron microscopy and (iii) the precise quantification of label down to 1 part-per-million and spanning several orders of magnitude. Here we review the basic elements of MIMS and describe new applications of MIMS to the quantitative study of metabolic processes including protein and nucleic acid synthesis in model organisms ranging from microbes to humans.
多同位素成像质谱（MIMS）是利用新型二次离子质谱仪（NanoSIMS）对细胞内稳定同位素标记物进行定量成像的新技术。该方法的优势在于：(i) 使用无毒稳定同位素标记物具有巨大优势；(ii) 高分辨率成像，接近通常的透射电子显微镜分辨率；(iii) 精确定量标记物，低至百万分之一，跨越几个数量级。本文综述了 MIMS 的基本要素，并描述了 MIMS 在定量研究代谢过程中的新应用，包括从微生物到人等模式生物的蛋白质和核酸合成。
