Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
PLoS One. 2013 May 7;8(5):e64178. doi: 10.1371/journal.pone.0064178. Print 2013.
Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) and multivariate statistical analysis were used to investigate the processing technology of Loquat (Eriobotrya japonica) leaf (pipaye, PPY). The differences in samples processed using different methods were revealed by unsupervised principal component analysis (PCA). In the scores plot of PCA, honey-processed PPY (PPPY), crude PPY (CPPY), and heated PPY (HPPY) were clearly discriminated. Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing. Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers. The method was further confirmed by a verification test with commercial PPY. The orthogonal array experiment revealed an optimized processing condition with 50% honey at 140°C for 20 min after 4 h of moistening time, a process that provides significant information for standardized production.
采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-QTOF/MS）和多元统计分析方法，研究了枇杷叶（批杷叶，PPY）的炮制工艺。采用无监督主成分分析（PCA）揭示了不同方法处理的样品之间的差异。在 PCA 的得分图中，蜜炙 PPY（PPPY）、生 PPY（CPPY）和热炙 PPY（HPPY）明显区分开来。此外，不同温度处理的样品也可以区分；事实上，我们的 PCA 结果表明，炮制过程中的温度很重要。正交偏最小二乘判别分析（OPLS-DA）发现了两个用于区分 PPPY 和 CPPY 的独特标记离子，可以作为潜在的化学标记物。该方法通过对商业 PPY 的验证试验进一步得到证实。正交数组实验揭示了一种优化的炮制条件，即 50%蜂蜜在 140°C 下处理 20 分钟，在湿润 4 小时后，该工艺为标准化生产提供了重要信息。
