Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
Oncol Rep. 2013 Jul;30(1):327-33. doi: 10.3892/or.2013.2452. Epub 2013 May 13.
microRNAs (miRNAs), small non-coding RNAs, have emerged as key regulators of a large number of genes. The present study aimed to explore novel biological functions of miR-330 in the human prostate cancer cell lines DU145 and PC3. We confirmed that miR-330 was downregulated and inversely correlated with specificity protein 1 (Sp1) expression. Overexpression of miR-330 by transfection of a chemically synthesized miR-330 mimic induced a reduction in expression levels of the Sp1 protein, accompanied by significant suppression of cellular migration and invasion capability. In addition, the Sp1-knockdown experiments presented similar phenomena. Finally, the luciferase reporter assay validated Sp1 as the direct target of miR-330. These findings indicate that miR-330 acts as an anti-metastatic miRNA in prostate cancer.
微小 RNA（miRNA）是一类小的非编码 RNA，已经成为大量基因的关键调控因子。本研究旨在探索 miR-330 在人前列腺癌细胞系 DU145 和 PC3 中的新的生物学功能。我们证实 miR-330 下调且与特异性蛋白 1（Sp1）表达呈负相关。通过转染化学合成的 miR-330 模拟物过表达 miR-330 会导致 Sp1 蛋白表达水平降低，同时显著抑制细胞迁移和侵袭能力。此外，Sp1 敲低实验也呈现出类似的现象。最后，荧光素酶报告基因检测验证了 Sp1 是 miR-330 的直接靶标。这些发现表明 miR-330 作为一种抗转移 miRNA 在前列腺癌中发挥作用。
