Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China.
Sci Rep. 2013;3:1843. doi: 10.1038/srep01843.
Although attempts have been made to reveal the relationships between bacteria and human health, little is known about the species and function of the microbial community associated with oral diseases. In this study, we report the sequencing of 16 metagenomic samples collected from dental swabs and plaques representing four periodontal states. Insights into the microbial community structure and the metabolic variation associated with periodontal health and disease were obtained. We observed a strong correlation between community structure and disease status, and described a core disease-associated community. A number of functional genes and metabolic pathways including bacterial chemotaxis and glycan biosynthesis were over-represented in the microbiomes of periodontal disease. A significant amount of novel species and genes were identified in the metagenomic assemblies. Our study enriches the understanding of the oral microbiome and sheds light on the contribution of microorganisms to the formation and succession of dental plaques and oral diseases.
尽管人们已经尝试揭示细菌与人类健康之间的关系，但对于与口腔疾病相关的微生物群落的物种和功能知之甚少。在本研究中，我们报告了从牙菌斑和牙菌斑中采集的 16 个宏基因组样本的测序结果，这些样本代表了四种牙周状态。我们获得了有关微生物群落结构和与牙周健康和疾病相关的代谢变化的见解。我们观察到群落结构与疾病状态之间存在很强的相关性，并描述了一个核心的疾病相关群落。在牙周病微生物组中，许多功能基因和代谢途径，包括细菌趋化性和聚糖生物合成，都被过度表达。在宏基因组组装中还鉴定出大量的新物种和基因。我们的研究丰富了对口腔微生物组的理解，并揭示了微生物对牙菌斑和口腔疾病形成和演替的贡献。
