Colciago Alessandra, Celotti Fabio, Casati Lavinia, Giancola Rinaldo, Castano Stefano M, Antonini Guido, Sacchi Maria Cristina, Negri-Cesi Paola
Department of Endocrinology, Pathophysiology and Applied Biology, University of Milano, Via Balzaretti 9, 20133 Milano, Italy.
Int J Biomed Sci. 2009 Dec;5(4):380-9.
PDGF is a major constituent of platelet rich plasma (PRP), responsible of chemotactic and possibly of mitogenic effects of PRP on osteoblasts. PDGF family includes 5 isoforms: PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, all expressed in platelets except PDGF-DD. Aim of this study was to analyze the effect of recombinant hPDGF-A, -AB, -B and -C, on migration and proliferation of a human osteoblastic cell line, SaOS-2. Preliminary observations on cell migration were also done in primary cultures of human osteoblasts. In vitro microchemotaxis and (3)H-thymidine mitogenic assays were used. While PDGF-AB is active at concentrations present in PRP, PDGF-AA and BB are chemotactic only at much higher doses. PDGF-C is totally inactive alone or together with the active isoforms. PDGF-AA, PDGF-BB and PDGF-C stimulate SaOS-2 proliferation only at the highest dose tested, while PDGF-AB is ineffective. Primary osteoblasts are less sensitive than SaOS-2 and progressively lose responsiveness with increasing passages in culture, in line with loss of cell differentiation. The different PDGF isoforms act differentially on osteoblasts, the-AB isoform appearing the major responsible of the PRP chemiotaxis. PDGF, at the concentrations present in PRP, does not affect cell proliferation.
血小板衍生生长因子（PDGF）是富血小板血浆（PRP）的主要成分，负责PRP对成骨细胞的趋化作用以及可能的促有丝分裂作用。PDGF家族包括5种异构体：PDGF-AA、PDGF-AB、PDGF-BB、PDGF-CC和PDGF-DD，除PDGF-DD外，所有异构体均在血小板中表达。本研究的目的是分析重组人PDGF-A、-AB、-B和-C对人成骨细胞系SaOS-2迁移和增殖的影响。还对人成骨细胞原代培养物进行了细胞迁移的初步观察。采用体外微量趋化性和³H-胸腺嘧啶核苷促有丝分裂试验。虽然PDGF-AB在PRP中的浓度下具有活性，但PDGF-AA和BB仅在高得多的剂量下才具有趋化作用。PDGF-C单独或与活性异构体一起时完全无活性。PDGF-AA、PDGF-BB和PDGF-C仅在测试的最高剂量下刺激SaOS-2增殖，而PDGF-AB无效。原代成骨细胞比SaOS-2敏感性低，并且随着培养传代次数增加逐渐失去反应性，这与细胞分化丧失一致。不同的PDGF异构体对成骨细胞的作用不同，AB异构体似乎是PRP趋化作用的主要原因。PRP中存在的浓度的PDGF不影响细胞增殖。
