Prabhu V Vinod, Guruvayoorappan C
Department of Biotechnology, Karunya University, Karunya Nagar, Tamil Nadu, India.
Asian Pac J Cancer Prev. 2013;14(3):1833-40. doi: 10.7314/apjcp.2013.14.3.1833.
Metastasis is one of the hallmarks of malignant neoplasms and is the leading cause of death in many cancer patients. A major challenge in cancer treatment is to find better ways to specifically target tumor metastasis. In this study, the anti-metastatic potential of the methanolic extract of Rhizophora apiculata (R.apiculata) was evaluated using the B16F-10 melanoma induced lung metastasis model in C57BL/6 mice. Metastasis was induced in C57BL/6 mice by injecting highly metastatic B16F-10 melanoma cells through the lateral tail vein. Simultaneous treatment with R.apiculata extract (10 mg/kg b.wt (intraperitoneal) significantly (p<0.01) inhibited pulmonary tumor nodule formation (41.1 %) and also increased the life span (survival rate) 107.3 % of metastatic tumor bearing animals. The administration of R.apiculata extract significantly (p<0.01) reduced biochemical parameters such as lung collagen hydroxyproline, hexosamine, uronic acid content, serum nitric oxide (NO), γ-glutamyl transpeptidase (GGT) and sialic acid levels when compared to metastasis controls. These results correlated with lung histopathology analysis of R.apiculata extract treated mice showing reduction in lung metastasis and tumor masses. Taken together, our findings support that R.apiculata extract could be used as a potential anti-metastasis agent against lung cancer.
转移是恶性肿瘤的标志之一，也是许多癌症患者的主要死因。癌症治疗中的一个主要挑战是找到更好的方法来特异性靶向肿瘤转移。在本研究中，使用C57BL/6小鼠的B16F - 10黑色素瘤诱导的肺转移模型评估了红海榄甲醇提取物（红海榄）的抗转移潜力。通过侧尾静脉注射高转移性B16F - 10黑色素瘤细胞在C57BL/6小鼠中诱导转移。同时用红海榄提取物（10 mg/kg体重（腹腔内））治疗可显著（p<0.01）抑制肺肿瘤结节形成（41.1%），并且还使转移性肿瘤携带动物的寿命（存活率）提高了107.3%。与转移对照组相比，给予红海榄提取物显著（p<0.01）降低了生化参数，如肺胶原羟脯氨酸、己糖胺、糖醛酸含量、血清一氧化氮（NO）、γ-谷氨酰转肽酶（GGT）和唾液酸水平。这些结果与对红海榄提取物处理的小鼠的肺组织病理学分析相关，显示肺转移和肿瘤块减少。综上所述，我们的研究结果支持红海榄提取物可作为一种潜在的抗肺癌转移剂。
