Preethi Korangath C, Siveen Kodappully S, Kuttan Ramadasan, Kuttan Girija
Department of Biochemistry, Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India.
Asian Pac J Cancer Prev. 2010;11(6):1773-9.
To determine the effect of a Calendula officinalis flower extract on lung metastasis by B16F-10 melanoma cells in C57BL/6 mice.
Male mice were injected with B16F-10 melanoma cells through the tail vein and simultaneously treated with C.officinalis flower extract. Parameters studied were lung tumor nodule count, life span of animals, gamma glutamyl transpeptidase activity, sialic acid, TNF-α, IL-1β, IL-6, IL-2, GM-CSF, VEGF and TIMP-1 levels in serum, and lung hydroxyproline, uronic acid and hexosamine levels, as well as histopathological features. Effects of C.officinalis on the expression of various genes involved in metastasis like matrix metalloproteases (MMPs), tissue inhibitor of metalloproteases (TIMPs), prolyl hydoxylase, lysyl oxidase, nm23, and proinflammatory cytokines were also investigated.
Simultaneous administration of C. officinalis extract to tumor bearing C57BL/6 mice reduced the lung tumor nodules by 74% with 43.3% increase in life span. Elevated levels of hydroxyproline, uronic acid, hexosamine, serum sialic acid and γ-glutamyl transpeptidase in the metastatic controls were found to be significantly lowered in the C. officinalis treated animals. The extract also inhibited expression of MMP-2, MMP-9, prolyl hydroxylase and lysyl oxidase and activated TIMP-1 and TIMP-2 and downregulated proinflammatory cytokines.
The present investigation indicated antimetastatic effects of Calendula officinalis flowers through the inhibition of key enzymes involved in processes of metastasis.
确定金盏花花提取物对C57BL/6小鼠B16F - 10黑色素瘤细胞肺转移的影响。
雄性小鼠经尾静脉注射B16F - 10黑色素瘤细胞，并同时用金盏花花提取物进行处理。研究的参数包括肺肿瘤结节计数、动物寿命、血清中γ - 谷氨酰转肽酶活性、唾液酸、肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6、白细胞介素-2、粒细胞巨噬细胞集落刺激因子、血管内皮生长因子和基质金属蛋白酶组织抑制因子-1水平，以及肺羟脯氨酸、糖醛酸和氨基己糖水平，还有组织病理学特征。还研究了金盏花对参与转移过程的各种基因表达的影响，如基质金属蛋白酶（MMPs）、基质金属蛋白酶组织抑制因子（TIMPs）、脯氨酰羟化酶、赖氨酰氧化酶、nm23和促炎细胞因子。
给荷瘤C57BL/6小鼠同时给予金盏花提取物，可使肺肿瘤结节减少74%，寿命延长43.3%。发现转移对照组中升高的羟脯氨酸、糖醛酸、氨基己糖、血清唾液酸和γ - 谷氨酰转肽酶水平在经金盏花处理的动物中显著降低。该提取物还抑制MMP - 2、MMP - 9、脯氨酰羟化酶和赖氨酰氧化酶的表达，激活TIMP - 1和TIMP - 2，并下调促炎细胞因子。
本研究表明金盏花花具有抗转移作用，其机制是抑制转移过程中涉及的关键酶。
