Subbaiya Ramasamy, Saravanan Muthupandian, Priya Andavar Raja, Shankar Konathala Ravi, Selvam Masilamani, Ovais Muhammad, Balajee Ramachandran, Barabadi Hamed
Department of Biotechnology, K.S. Rangasamy College of Technology, Namakkal (District), Tiruchengode 637 215, Tamil Nadu, India.
Department of Medical Microbiology and Immunology, Institute of Biomedical Sciences, College of Health Science, Mekelle University, Mekelle 1871, Ethiopia.
IET Nanobiotechnol. 2017 Dec;11(8):965-972. doi: 10.1049/iet-nbt.2016.0222.
Silver nanoparticles (AgNPs) have been undeniable for its antimicrobial activity while its antitumour potential is still limited. Therefore, the present study focused on determining cytotoxic effects of AgNPs on Michigan cancer foundation-7 (MCF-7) breast cancer cells and its corresponding mechanism of cell death. Herein, the authors developed a bio-reduction method for AgNPs synthesis using actinomycetes isolated from marine soil sample. The isolated strain was identified by 16s ribotyping method and it was found to be . Furthermore, the synthesised AgNPs were characterised by various bio-analytical techniques such as ultraviolet-visible spectrophotometer, atomic force microscopy, transmission electron microscopy, Fourier transform infra-red spectroscopy, and X-ray diffraction. Moreover, the results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay reveals 44.51 µg of AgNPs to have profound inhibition of cancer cell growth; furthermore, the inhibition of MCF-7 breast cancer cell line was found to be dose dependent on treatment with AgNPs. Acridine orange and ethidium bromide double staining methods were performed for cell morphological analysis. The present results showed that biosynthesised AgNPs might be emerging alternative biomaterials for human breast cancer therapy.
银纳米颗粒（AgNPs）的抗菌活性是不可否认的，但其抗肿瘤潜力仍然有限。因此，本研究着重于确定AgNPs对密歇根癌症基金会-7（MCF-7）乳腺癌细胞的细胞毒性作用及其相应的细胞死亡机制。在此，作者开发了一种利用从海洋土壤样品中分离出的放线菌进行AgNPs合成的生物还原方法。通过16s核糖体分型法对分离出的菌株进行了鉴定，发现它是 。此外，通过各种生物分析技术对合成的AgNPs进行了表征，如紫外可见分光光度计、原子力显微镜、透射电子显微镜、傅里叶变换红外光谱和X射线衍射。此外，3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐试验结果显示，44.51μg的AgNPs对癌细胞生长有显著抑制作用；此外，发现用AgNPs处理对MCF-7乳腺癌细胞系的抑制作用呈剂量依赖性。采用吖啶橙和溴化乙锭双重染色法进行细胞形态分析。目前的结果表明，生物合成的AgNPs可能成为人类乳腺癌治疗中新兴的替代生物材料。
