体细胞重编程的机制和模型。
Mechanisms and models of somatic cell reprogramming.
机构信息
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
出版信息
Nat Rev Genet. 2013 Jun;14(6):427-39. doi: 10.1038/nrg3473.
Abstract
Conversion of somatic cells to pluripotency by defined factors is a long and complex process that yields embryonic-stem-cell-like cells that vary in their developmental potential. To improve the quality of resulting induced pluripotent stem cells (iPSCs), which is important for potential therapeutic applications, and to address fundamental questions about control of cell identity, molecular mechanisms of the reprogramming process must be understood. Here we discuss recent discoveries regarding the role of reprogramming factors in remodelling the genome, including new insights into the function of MYC, and describe the different phases, markers and emerging models of reprogramming.
摘要
通过定义因子将体细胞转化为多能性是一个漫长而复杂的过程，会产生在发育潜能上存在差异的胚胎干细胞样细胞。为了提高诱导多能干细胞（iPSC）的质量，这对于潜在的治疗应用很重要，并且为了解决关于细胞身份控制的基本问题，必须了解重编程过程的分子机制。在这里，我们讨论了关于重编程因子在重塑基因组中的作用的最新发现，包括对 MYC 功能的新见解，并描述了重编程的不同阶段、标记物和新兴模型。
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