Gastroenterology Department, Federal University of São Paulo, UNIFESP/EPM, Brazil.
Cell Immunol. 2013 Mar;282(1):66-70. doi: 10.1016/j.cellimm.2013.04.004. Epub 2013 Apr 23.
Serum calprotectin is elevated in patients with inflammatory bowel disease (IBD). Whether it correlates other markers of disease activity is unknown. The aim of this study was to correlate serum calprotectin with biochemical and histological measures of intestinal inflammation.
TNBS colitis was induced in wistar rats, and serial blood samples were collected at 0, 3, and 12 days. Animals were subsequently sacrificed for pathological evaluation at day 12. Serum calprotectin and cytokines were measured by ELISA. Pathologic changes were classified at the macroscopic and microscopic levels.
TNBS colitis induced elevated serum calprotectin, TNF and IL-6 within 24 h. Levels of serum calprotectin remained elevated in parallel to persistence of loose stool and weight loss to day 12. Serum calprotectin levels correlated with serum levels of TNF-α and IL6 (p < 0.001), but not CRP. Animals with liquid stool had significantly higher levels of serum calprotectin than control animals. There was a correlation between macroscopic colitis scores, and levels of serum calprotectin.
Serum calprotectin levels correlate with biochemical and histological markers of inflammation in TNBS colitis. This biomarker may have potential for diagnostic use in patients with IBD.
血清钙卫蛋白在炎症性肠病（IBD）患者中升高。目前尚不清楚它是否与其他疾病活动标志物相关。本研究旨在探讨血清钙卫蛋白与肠道炎症的生化和组织学指标的相关性。
采用 TNBS 结肠炎诱导 Wistar 大鼠，并在 0、3 和 12 天采集连续血样。动物在第 12 天进行病理评估时被处死。通过 ELISA 法测定血清钙卫蛋白和细胞因子。在宏观和微观水平上对病理变化进行分类。
TNBS 结肠炎诱导的血清钙卫蛋白、TNF 和 IL-6 在 24 小时内升高。血清钙卫蛋白水平在持续出现稀便和体重减轻至第 12 天期间仍保持升高。血清钙卫蛋白水平与 TNF-α和 IL6 血清水平相关（p<0.001），但与 CRP 无关。有液体便的动物血清钙卫蛋白水平明显高于对照组动物。血清钙卫蛋白水平与宏观结肠炎评分之间存在相关性。
血清钙卫蛋白水平与 TNBS 结肠炎的生化和组织学炎症标志物相关。这种生物标志物可能在 IBD 患者的诊断中有应用潜力。
