阿尔及利亚耐多药鲍曼不动杆菌临床分离株的抗生素耐药决定因子。
Antibiotic resistance determinants of multidrug-resistant Acinetobacter baumannii clinical isolates in Algeria.
机构信息
Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE) CNRS-IRD INSERM 7278, Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.
出版信息
Diagn Microbiol Infect Dis. 2013 Aug;76(4):529-31. doi: 10.1016/j.diagmicrobio.2013.04.009. Epub 2013 May 18.
Antibiotic susceptibility testing was performed on 71 Acinetobacter baumannii clinical isolates, and presence of antibiotic resistance genes was screened for by PCR amplification and sequencing. Resistance rates were very high for aminoglycosides (22-80%), fluoroquinolones (>90%), and cephalosporins (>90%) but remained low for rifampin (2.8%) or null for colistin. Antibiotic resistance encoding genes detected were as follows: blaTEM-128 gene (74.6%), aph(3')-VI (50.7 %), aadA (63.4%), ant(2″)-I (14.1%), aac(3)-Ia (91.1%), aac(6')-Ib (4.2%), mutation Ser83Leu in gyrA (94.4%), double mutations Ser83Leu and Ser80Leu (or Ser84Leu) in gyrA and parC (69.0%), and mutation I581N in RRDR of the rpoB gene.
对 71 株鲍曼不动杆菌临床分离株进行了抗生素药敏试验，通过 PCR 扩增和测序筛选了抗生素耐药基因。氨基糖苷类（22-80%）、氟喹诺酮类（>90%）和头孢菌素类（>90%）的耐药率非常高，但利福平（2.8%）的耐药率较低或对多粘菌素耐药。检测到的抗生素耐药基因编码如下：blaTEM-128 基因（74.6%）、aph(3')-VI（50.7%）、aadA（63.4%）、ant(2″)-I（14.1%）、aac(3)-Ia（91.1%）、aac(6')-Ib（4.2%）、gyrA 中的 Ser83Leu 突变（94.4%）、gyrA 和 parC 中的 Ser83Leu 和 Ser80Leu（或 Ser84Leu）双重突变（69.0%）和 rpoB 基因 RRDR 中的 I581N 突变。
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