Kwon Ho Jang, Lim Myung Ho
Environmental Health Center, Dankook Medical Hospital, Cheonan, South Korea.
Genet Test Mol Biomarkers. 2013 Jul;17(7):529-34. doi: 10.1089/gtmb.2013.0072. Epub 2013 May 21.
Attention-deficit hyperactivity disorder (ADHD) is a common disorder of the school-age population. ADHD is familial, and genetic studies estimate heritability at 80%-90%. The aim of the present study was to investigate the association between the genetic type and alleles for the dopamine beta-hydroxylase (DBH) gene in Korean children with ADHD. The sample consisted of 142 ADHD children and 139 control children. We diagnosed ADHD according to DSM-IV. ADHD symptoms were evaluated with the Conners' Parent Rating Scales and Dupaul Parent ADHD Rating Scales. Blood samples were taken from the 281 subjects; DNA was extracted from blood lymphocytes, and polymerase chain reaction was performed for the DBH polymorphism. The alleles and genotype frequencies were compared using the chi-square test. We compared the allele and genotype frequencies of the DBH gene polymorphism in the ADHD and control groups. This study showed that there was a significant correlation among the frequencies of rs1611115 (odds ratio=0.64, 95% confidence interval=0.42-0.97, p=0.034) of the alleles of DBH, but the final conclusions are not definite. Follow-up studies with larger patient or pure subgroups are expected. These results suggested that DBH might be related to ADHD symptoms.
注意力缺陷多动障碍（ADHD）是学龄儿童中的一种常见疾病。ADHD具有家族性，遗传研究估计其遗传度在80%-90%。本研究的目的是调查韩国ADHD儿童中多巴胺β-羟化酶（DBH）基因的基因类型与等位基因之间的关联。样本包括142名ADHD儿童和139名对照儿童。我们根据《精神疾病诊断与统计手册》第四版（DSM-IV）诊断ADHD。使用康纳斯父母评定量表和杜保罗父母ADHD评定量表评估ADHD症状。从281名受试者采集血样；从血液淋巴细胞中提取DNA，并对DBH多态性进行聚合酶链反应。使用卡方检验比较等位基因和基因型频率。我们比较了ADHD组和对照组中DBH基因多态性的等位基因和基因型频率。本研究表明，DBH等位基因的rs1611115频率之间存在显著相关性（优势比=0.64，95%置信区间=0.42-0.97，p=0.034），但最终结论并不确定。预计将对更大的患者群体或纯亚组进行后续研究。这些结果表明DBH可能与ADHD症状有关。
