International Environmental Analysis and Education Center, Gwangju Institute of Science and Technology, 123 Cheomdan-Gwagiro, Bukgu, Gwangju, 500-712, Republic of Korea,
World J Microbiol Biotechnol. 2013 Nov;29(11):2115-24. doi: 10.1007/s11274-013-1376-3. Epub 2013 May 22.
Surface water samples were collected from rivers which fed into large urban areas within Vietnam, Indonesia, Cambodia, and Thailand and were processed to enumerate Escherichia coli. Selected isolates were further characterized using PCR to detect the presence of specific virulence genes. Analyzing the four countries together, the approximate mean cfu/100 ml for E. coli counts in the dry season were log 4.3, while counts in the wet season were log 2.8. Of the 564 E. coli isolates screened for the presence of pathogenic genes, 3.9 % possessed at least one virulence gene. The most common pathogenic types found were Shiga toxin-producing E. coli isolates. These results reinforce the importance of monitoring urban surface waters for fecal contamination, that E. coli in these water environments may serve as opportunistic pathogens, and may help in determining the impact water usage from these rivers have on the public health of urban populations in Southeast Asia.
采集了来自越南、印度尼西亚、柬埔寨和泰国的流入大型城市的河流的地表水样本，并对其进行处理以计数大肠杆菌。选择的分离株进一步通过 PCR 进行特征分析，以检测特定毒力基因的存在。将这四个国家一起分析，旱季大肠杆菌计数的近似平均值为 4.3 对数，而雨季的计数为 2.8 对数。在筛选存在致病基因的 564 株大肠杆菌分离株中，有 3.9%至少携带一种毒力基因。发现的最常见的致病类型是产志贺毒素大肠杆菌分离株。这些结果强调了监测城市地表水粪便污染的重要性，这些水环境中的大肠杆菌可能是机会性病原体，并可能有助于确定这些河流的用水对东南亚城市人口公共健康的影响。
