Cell Cycle Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
Open Biol. 2013 May 22;3(5):130053. doi: 10.1098/rsob.130053.
To identify near complete sets of genes required for the cell cycle and cell shape, we have visually screened a genome-wide gene deletion library of 4843 fission yeast deletion mutants (95.7% of total protein encoding genes) for their effects on these processes. A total of 513 genes have been identified as being required for cell cycle progression, 276 of which have not been previously described as cell cycle genes. Deletions of a further 333 genes lead to specific alterations in cell shape and another 524 genes result in generally misshapen cells. Here, we provide the first eukaryotic resource of gene deletions, which describes a near genome-wide set of genes required for the cell cycle and cell shape.
为了鉴定细胞周期和细胞形态所必需的近完整基因集，我们对一个包含 4843 个裂殖酵母缺失突变体（编码蛋白的基因总数的 95.7%）的全基因组基因缺失文库进行了可视化筛选，以观察它们对这些过程的影响。共有 513 个基因被鉴定为细胞周期进展所必需的，其中 276 个以前没有被描述为细胞周期基因。另外 333 个基因的缺失导致细胞形态的特定改变，还有 524 个基因导致细胞普遍畸形。在这里，我们提供了第一个真核生物基因缺失资源，它描述了一个近全基因组的细胞周期和细胞形态所必需的基因集。
