Service de Microbiologie, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Paris, France.
J Clin Microbiol. 2013 Aug;51(8):2713-6. doi: 10.1128/JCM.00077-13. Epub 2013 May 22.
Overall, 2,337 rectal screening samples (RSSs) were seeded by using the Wasp instrument for automated microbiological processing with five media for detection of extended-spectrum β-lactamase (ESBL): CHROMagar, ChromID, Brilliance, BD Drigalski, and HEGP media. Of 354 RSSs harboring ESBL-producing isolates, 89.3% were found to be positive on all media. Sensitivity and specificity ranged from 95.5 to 98.3% and from 57.9 to 72.3%, respectively. No medium was perfectly ESBL selective, and non-ESBL-producing strains were mainly Enterobacteriaceae overproducing AmpC β-lactamase and nonfermenting Gram-negative bacilli, mostly Pseudomonas aeruginosa.
总体而言，使用 Wasp 仪器对 2337 份直肠筛查样本（RSS）进行了播种，这些 RSS 采用五种用于检测扩展谱β-内酰胺酶（ESBL）的培养基进行了自动化微生物处理：CHROMagar、ChromID、Brilliance、BD Drigalski 和 HEGP 培养基。在 354 份携带产 ESBL 分离株的 RSS 中，所有培养基均有 89.3%呈阳性。敏感性和特异性分别为 95.5%至 98.3%和 57.9%至 72.3%。没有一种培养基对 ESBL 具有完全的选择性，而不产 ESBL 的菌株主要是产 AmpCβ-内酰胺酶的肠杆菌科和非发酵革兰氏阴性杆菌，主要是铜绿假单胞菌。
