Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Tehran, Iran.
Sci Rep. 2022 Aug 17;12(1):13981. doi: 10.1038/s41598-022-18298-2.
The purpose of this study was to investigate the effects of rosiglitazone on ram semen after cryopreservation on the quality of thawed sperm. Sperm motility, membrane functionality, viability, total abnormality, acrosome membrane integrity, mitochondrial activity, reactive oxygen species production, ATP content and apoptotic features were assessed after thawing. Rosiglitazone at concentration of 60 µM resulted in the highest (P < 0.05) total motility, progressive motility and straight-line velocity. The percentages of average path velocity and curvilinear velocity were greater in the 60 µM group. Different concentrations of rosiglitazone did not have significant effects on amplitude of the lateral head displacement, linearity and straightness. The highest amounts of membrane functionality and mitochondrial activity after freeze-thawing were observed in groups containing 60 µM. By increasing the rosiglitazone level to 80 µM, no positive effect was observed in most of the evaluated parameters. The lowest ROS concentration was recorded in 60 µM rosiglitazone group (P < 0.05). The group containing 60 µM rosiglitazone also produced the lowest significant percentage of apoptosis-like changes and dead sperm. A greater (P < 0.05) percentage of acrosome integrity in frozen-thawed spermatozoa was observed in the 60 µM rosiglitazone group. There was no significant difference between 40 and 60 µM rosiglitazone in intact acrosome of ram thawed semen. The result showed that supplementation in ram semen extender with rosiglitazone had a positive role in the regulation of ram sperm motility and had strong protective effect on the sperm membrane and acrosome integrity.
本研究旨在探讨罗格列酮对冷冻保存后公羊精液质量的影响。解冻后评估精子活力、膜功能、活力、总畸形率、顶体膜完整性、线粒体活性、活性氧产生、ATP 含量和凋亡特征。浓度为 60µM 的罗格列酮导致总活力、前向运动活力和直线速度最高（P<0.05）。60µM 组的平均路径速度和曲线速度百分比更大。不同浓度的罗格列酮对侧向头部位移幅度、线性和直线性没有显著影响。冷冻解冻后膜功能和线粒体活性最高的是含有 60µM 罗格列酮的组。将罗格列酮水平提高到 80µM 时，大多数评估参数没有观察到积极影响。ROS 浓度最低的是 60µM 罗格列酮组（P<0.05）。含有 60µM 罗格列酮的组也产生了最低的凋亡样变化和死精子的显著百分比。冷冻解冻精子中顶体完整率在 60µM 罗格列酮组中观察到更大（P<0.05）。40 和 60µM 罗格列酮之间在公羊解冻精液中完整顶体没有显著差异。结果表明，在公羊精液稀释剂中添加罗格列酮对调节公羊精子活力具有积极作用，并对精子膜和顶体完整性具有很强的保护作用。
