Akbulut Sevtap, Altintaş Hande, Berk Derya, Sezen Ozan Seymen, Barisik Nagehan Ozdemir
Department of Otolaryngology Head and Neck Surgery, Dr. Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey.
Int J Pediatr Otorhinolaryngol. 2013 Jul;77(7):1147-51. doi: 10.1016/j.ijporl.2013.04.027. Epub 2013 May 22.
Everolimus is an immunosuppressant and an antiproliferative macrolide agent which is used for coronary stent coating for local inhibition of in-stent restenosis. The aim of this study was to evaluate the effect of everolimus-coated tympanostomy tubes in rat tympanic membrane.
Prospective controlled animal study.
Twenty-one Sprague-Dawley rats were included in the study. They were bilaterally myringotomized. An everolimus-coated fluoroplastic tympanostomy tube was inserted into one ear of each of the rats (everolimus-coated TT Group; n=21) and a standard fluoroplastic tympanostomy tube was applied to the contralateral ear of the same rats (Standard TT Group; n=21). Tympanic membranes and tubes were routinely examined otomicroscopically every other day for two months. Then the membranes were harvested and evaluated histologically.
In everolimus-coated tubes, the tube obstruction was found to be significantly lower than in standard tubes (p<0.05). The tube extrusion rate was less for everolimus-coated tubes than for standard tubes, but no statistically significant difference was observed between the two tubes. There was no statistically significant difference in the incidence of otorrhea between two tubes. Histopathological examination under light microscope revealed that everolimus-coated tubes resulted in less fibrosis and less inflammation in tympanic membranes than standard tubes (p<0.01).
The study shows the potential role of everolimus-coating of tympanostomy tubes in reducing tube obstruction. In addition, inflammatory reaction and fibrosis were observed to be significantly less with these tubes.
依维莫司是一种免疫抑制剂和抗增殖大环内酯类药物，用于冠状动脉支架涂层以局部抑制支架内再狭窄。本研究的目的是评估依维莫司涂层鼓膜造孔管在大鼠鼓膜中的作用。
前瞻性对照动物研究。
21只Sprague-Dawley大鼠纳入本研究。对它们进行双侧鼓膜切开术。将一根依维莫司涂层氟塑料鼓膜造孔管插入每只大鼠的一只耳朵（依维莫司涂层TT组；n = 21），并将一根标准氟塑料鼓膜造孔管应用于同一只大鼠的对侧耳朵（标准TT组；n = 21）。每隔一天用耳显微镜对鼓膜和管子进行常规检查，持续两个月。然后取出鼓膜并进行组织学评估。
在依维莫司涂层管中，发现管阻塞明显低于标准管（p<0.05）。依维莫司涂层管的管挤出率低于标准管，但两组管之间未观察到统计学上的显著差异。两组管之间耳漏发生率无统计学显著差异。光学显微镜下的组织病理学检查显示，依维莫司涂层管导致鼓膜的纤维化和炎症比标准管少（p<0.01）。
该研究显示了依维莫司涂层鼓膜造孔管在减少管阻塞方面的潜在作用。此外，观察到这些管子的炎症反应和纤维化明显较少。
