Feyerabend C, Russell M A
J Pharm Pharmacol. 1979 Feb;31(2):73-6. doi: 10.1111/j.2042-7158.1979.tb13435.x.
A rapid and sensitive method for the measurement of nicotine in plasma, urine, saliva and breast milk is described. An internal standard (quinoline) is added to the samples and these are made alkaline and extracted with diethyl ether. The solvent is evaporated to small bulk and extracted with dilute acid which is then made alkaline. The nicotine is finally extracted into butyl acetate and an aliquot of this extract is injected onto a gas-chromatograph fitted with a nitrogen detector. Quantitation relies on comparison of peak areas and the calibration curve is linear over the concentration range 0.5 to 100 ng ml-1. Nicotine concentrations as low as 0.1 ng ml-1 can be measured. In addition, a micro-method is described which requires only 100 microliter of sample and yields an accurate result in 5 min.
本文描述了一种快速、灵敏的测定血浆、尿液、唾液和母乳中尼古丁的方法。向样品中加入内标物（喹啉），使其呈碱性，然后用乙醚萃取。将溶剂蒸发至小体积，再用稀酸萃取，接着将酸液调至碱性。尼古丁最终被萃取到乙酸丁酯中，取该萃取液的一份注入配有氮检测器的气相色谱仪中。定量分析依靠峰面积比较，校准曲线在0.5至100 ng ml-1的浓度范围内呈线性。最低可检测到0.1 ng ml-1的尼古丁浓度。此外，还描述了一种微量方法，该方法仅需100微升样品，并能在5分钟内得出准确结果。
