State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Environmental Protection Research Institute of Light Industry, Beijing 100089, PR China.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):3994-3999. doi: 10.1099/ijs.0.051938-0. Epub 2013 May 24.
A novel pyrene-degrading, Gram-negative bacterium, designated strain P-4(T), was isolated from a polycyclic aromatic hydrocarbon-degrading enrichment of polluted soils from a coking chemical plant. Cells of strain P-4(T) were non-motile rods. Strain P-4(T) grew at 15-45 °C (optimum, 37 °C), pH 6.0-10.0 (optimum, pH 8.5) and 0-4 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence showed that strain P-4(T) was related phylogenetically to members of the genus Parapedobacter, with sequence similarity of 93.7-95.1 %. The cellular fatty acids of strain P-4(T) were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 0 3-OH, summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0), anteiso-C15 : 0, iso-C15 : 0 3-OH, C16 : 0, iso-C15 : 1 G, C16 : 0 3-OH and C17 : 0 2-OH. Cells contained menaquinone 7 as the major quinone. The polyamine of strain P-4(T) was homospermidine, and the main polar lipids were phosphatidylethanolamine and a sphingolipid. The G+C content of the DNA was 45.4 mol%. Strain P-4(T) showed a range of phenotypic characteristics that differentiated it from previously recognized Parapedobacter species, particularly its ability to use pyrene as a sole carbon source for growth and its alkaline optimal pH for growth (pH 8.5). On the basis of these results, it is concluded that strain P-4(T) represents a novel species of the genus Parapedobacter, for which the name Parapedobacter pyrenivorans (type strain P-4(T) = NBRC 109113(T) = CGMCC 1.12195(T)) is proposed. An emended description of the genus Parapedobacter is also provided.
一株新的降解芘的革兰氏阴性菌，被命名为 P-4(T) 菌株，是从焦化化工厂污染土壤的多环芳烃降解富集物中分离得到的。P-4(T) 菌株的细胞为非运动棒状。P-4(T) 菌株在 15-45°C（最佳温度为 37°C）、pH6.0-10.0（最佳 pH8.5）和 0-4%（w/v）NaCl 下生长。16S rRNA 基因序列分析表明，P-4(T) 菌株与 Parapedobacter 属的成员在系统发育上具有亲缘关系，序列相似性为 93.7-95.1%。P-4(T) 菌株的细胞脂肪酸为 iso-C15:0、总和特征 3（C16:1ω7c 和/或 C16:1ω6c）、iso-C17:0 3-OH、总和特征 9（iso-C17:1ω9c 和/或 10-甲基 C16:0）、anteiso-C15:0、iso-C15:0 3-OH、C16:0、iso-C15:1 G、C16:0 3-OH 和 C17:0 2-OH。细胞中含有menaquinone 7 作为主要醌。P-4(T) 菌株的多胺为 homospermidine，主要极性脂为磷脂酰乙醇胺和鞘脂。DNA 的 G+C 含量为 45.4 mol%。P-4(T) 菌株表现出一系列表型特征，使其与先前鉴定的 Parapedobacter 种区分开来，特别是其能够将芘作为生长的唯一碳源和生长的碱性最佳 pH（pH8.5）。基于这些结果，我们得出结论，P-4(T) 菌株代表 Parapedobacter 属的一个新种，建议将其命名为 Parapedobacter pyrenivorans（模式菌株 P-4(T)=NBRC 109113(T)=CGMCC 1.12195(T)）。还提供了对 Parapedobacter 属的修订描述。
