Agricultural Microbiology Division, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA), Wanju-gun, Jeollabuk-do, 55365, Republic of Korea.
National Institute of Horticultural and Herbal Science (NIHHS), RDA, Jeju 63240, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Oct;67(10):3728-3732. doi: 10.1099/ijsem.0.002162. Epub 2017 Sep 12.
A novel Gram-stain-negative bacterial strain, designated T16R-256, was isolated from the rhizosphere soil of tomato plants grown in a greenhouse in Yecheon-gun, Gyeongsangbuk-do, Republic of Korea and characterized using polyphasic taxonomy. Cells were aerobic, non-flagellated and rod-shaped. Colonies were light yellow, convex and round. The strain grew in the temperature range of 15-37 °C (optimally at 28-30 °C) and pH range of 7.0-9.0 (optimally at 7.0-8.0) and in 4 % NaCl (w/v). A comparison of 16S rRNA gene sequences showed that strain T16R-256 is a member of the genus Parapedobacter, exhibiting high sequence similarity with Parapedobacter pyrenivorans P-4 (94.2 %), Parapedobacter indicus RK1 (93.7 %), Parapedobacter koreensis Jip14 (93.7 %), Parapedobacter luteus 4M29 (93.6 %) and Parapedobacter soli DCY14 (93.4 %). The major polar lipids were phosphatidylethanolamine, sphingolipid, one aminophospholipid, two aminolipids and three unknown lipids. The major fatty acids (>10 % of the total fatty acids) were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 0 2-OH/C16 : 1ω7c. Strain T16R-256 contained MK-7 as the predominant respiratory quinone and homospermidine as the major polyamine. The genomic DNA G+C content of the type strain was 55.5 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain T16R-256 should be designated as representative of a novel species of the genus Parapedobacter, for which the name Parapedobacter lycopersici sp. nov. is proposed. The type strain is T16R-256 (=KACC 18788=JCM 31602).
一株新的革兰氏阴性细菌菌株，被命名为 T16R-256，从韩国庆尚北道义川县温室中种植的番茄根际土壤中分离得到，并通过多相分类学进行了特征描述。细胞为需氧、无鞭毛和杆状。菌落呈浅黄色、凸起和圆形。该菌株在 15-37°C 的温度范围（最佳温度为 28-30°C）和 pH 值范围为 7.0-9.0（最佳 pH 值为 7.0-8.0）以及 4%的 NaCl（w/v）中生长。16S rRNA 基因序列比较表明，菌株 T16R-256 是 Parapedobacter 属的成员，与 Parapedobacter pyrenivorans P-4（94.2%）、Parapedobacter indicus RK1（93.7%）、Parapedobacter koreensis Jip14（93.7%）、Parapedobacter luteus 4M29（93.6%）和 Parapedobacter soli DCY14（93.4%）的序列相似度较高。主要极性脂为磷脂酰乙醇胺、神经酰胺、一种氨基磷脂、两种氨基脂和三种未知脂。主要脂肪酸（占总脂肪酸的>10%）为 iso-C15:0、iso-C17:0 3-OH 和 iso-C15:0 2-OH/C16:1ω7c。菌株 T16R-256 含有 MK-7 作为主要呼吸醌和腐胺作为主要多胺。模式株的基因组 DNA G+C 含量为 55.5mol%。基于表型、化学分类和系统发育数据，菌株 T16R-256 应被指定为 Parapedobacter 属的一个新种的代表，该种被命名为 Parapedobacter lycopersici sp. nov.。模式株为 T16R-256（=KACC 18788=JCM 31602）。
