Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
ICAR-Indian Institute of Soil Science, Bhopal 462038, Madhya Pradesh, India.
Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):129-134. doi: 10.1099/ijs.0.069104-0. Epub 2014 Oct 8.
Strain RK1(T), a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium was isolated from a hexachlorocyclohexane (HCH) dumpsite, Lucknow, India. 16S rRNA gene sequence analysis revealed that strain RK1(T) belongs to the family Sphingobacteriaceae and showed highest sequence similarity to Parapedobacter koreensis Jip14(T) (95.63%). The major cellular fatty acids of strain RK1(T) were iso-C15:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c), iso-C17:0 3-OH, summed feature 9 (10-methyl C16:0 and/or iso-C17:1ω9c), iso-C15:0 3-OH and C16 : 0. The major respiratory pigment and polyamine of RK1(T) were menaquinone (MK-7) and homospermidine, respectively. The main polar lipids were phosphatidylethanolamine and sphingolipid. The G+C content of the DNA was 44.5 mol%. The results of physiological and biochemical tests and 16S rRNA sequence analysis clearly demonstrated that strain RK1(T) represents a novel species of the genus Parapedobacter, for which the name Parapedobacter indicus sp. nov. is proposed. The type strain is RK1(T) ( = DSM 28470(T) =MCC 2546(T)).
菌株 RK1(T)，革兰氏阴性、不产芽孢、杆状、非运动的细菌，从印度勒克瑙的六氯环己烷（HCH）倾倒场中分离得到。16S rRNA 基因序列分析表明，菌株 RK1(T)属于鞘脂杆菌科，与 Parapedobacter koreensis Jip14(T)（95.63%）的序列相似度最高。菌株 RK1(T)的主要细胞脂肪酸为 iso-C15:0、特征性脂肪酸 3（C16:1ω7c 和/或 C16:1ω6c）、iso-C17:0 3-OH、特征性脂肪酸 9（10-甲基 C16:0 和/或 iso-C17:1ω9c）、iso-C15:0 3-OH 和 C16 : 0。菌株 RK1(T)的主要呼吸色素和多胺分别为menaquinone（MK-7）和homospermidine。主要极性脂为磷脂酰乙醇胺和鞘脂。DNA 的 G+C 含量为 44.5 mol%。生理生化试验和 16S rRNA 序列分析结果清楚地表明，菌株 RK1(T)代表了 Parapedobacter 属的一个新种，因此提议将其命名为 Parapedobacter indicus sp. nov.。模式菌株为 RK1(T)（=DSM 28470(T)=MCC 2546(T)）。
