Liu Lei, Li Lubin, Song Zheng, Wang Shiyan, Zhang Jun, Zhang Xiaoxia, Sun Qiwu
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, PR China.
Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2148-2152. doi: 10.1099/ijsem.0.001911. Epub 2017 Jul 13.
Strain N5SSJ16T, a Gram-negative-staining, non-spore-forming, rod-shaped, non-motile bacterium, was isolated from Haloxylon ammodendron stems. The strain grew in the presence of 0-4 % (w/v) NaCl (optimum growth in the absence of NaCl), at pH 7-9 (optimum: pH 8) and at 12-50 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N5SSJ16T was related phylogenetically to Parapedobacter composti 4M40T (96.5 %) and Parapedobacter luteus 4M29T (95.9 %). The cellular fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), summed feature 9 (C16 : 0 10-methyl and/or iso-C17 : 1ω9c) and iso-C17 : 0 3-OH. MK-7 was the respiratory quinone. The main polar lipids were phosphatidylethanolamine, unidentified aminophospholipid and two unknown lipids. The G+C content of the DNA was 45.8 mol%. On the basis of chemotaxonomic, phylogenetic and phenotypic evidence, strain N5SSJ16T represents a novel species of the genus Parapedobacter, for which the name Parapedobacter deserti sp. nov. is proposed. The type strain is N5SSJ16T (=ACCC 19928T=KCTC 52416T).
菌株N5SSJ16T是一种革兰氏阴性、不形成芽孢、杆状、无运动性的细菌，从梭梭茎中分离得到。该菌株在0-4%（w/v）NaCl存在的情况下生长（在无NaCl时生长最佳），pH值为7-9（最适pH值：8），温度为12-50°C。基于16S rRNA基因序列的系统发育分析表明，菌株N5SSJ16T在系统发育上与堆肥副帕拉杆菌4M40T（96.5%）和黄色副帕拉杆菌4M29T（95.9%）相关。细胞脂肪酸为异C15:0、特征性总和3（C16:1ω7c和/或C16:1ω6c）、特征性总和9（C16:0 10-甲基和/或异C17:1ω9c）和异C17:0 3-OH。MK-7是呼吸醌。主要极性脂质是磷脂酰乙醇胺、未鉴定的氨基磷脂和两种未知脂质。DNA的G+C含量为45.8 mol%。基于化学分类、系统发育和表型证据，菌株N5SSJ16T代表副帕拉杆菌属的一个新物种，为此提出了新名称沙漠副帕拉杆菌（Parapedobacter deserti sp. nov.）。模式菌株为N5SSJ16T（=ACCC 19928T=KCTC 52416T）。
