Wang Rui, Zheng Yan-Yan, Liu Zi-Xuan, Zhang Xuan, Liao You-Jun, Yu Ya-Jing, Li Ai-Hua
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.
Antonie Van Leeuwenhoek. 2025 Jul 14;118(8):112. doi: 10.1007/s10482-025-02124-6.
A Gram-stain-negative, aerobic, rod-shaped bacterium strain, designated as 4-7-4, was isolated from the padding of a fermentation bed. Phylogenetic analyses based on 16S rRNA gene indicated that it belonged to the genus Parapedobacter and exhibited the closest relationship to P. soli KCTC 12984, with a similarity of 96.03%. The average nucleotide identity and digital DNA-DNA hybridization between strain 4-7-4 and P. soli KCTC 12984 were 78.6% and 21.6% respectively, both of which were below the thresholds for delineating species, supporting it being a novel species of Parapedobacter. The major polar lipids included phosphatidylethanolamine, unidentified aminolipid and four unidentified lipids. The predominant respiratory quinone was MK-7. The major cellular fatty acids were summed feature 3 (C ω7c and/or C ω6c) and iso-C. The genome of strain 4-7-4 was 4890729 bp, and the genomic DNA G + C content was 49.8%. In summary, strain 4-7-4 was identified as a novel species of Parapedobacter, for which the name Parapedobacter flavus sp. nov. was proposed. The type strain was 4-7-4 (=CGMCC1.10938 = KCTC 92351).
从发酵床的垫料中分离出一株革兰氏阴性、需氧、杆状细菌菌株，命名为4-7-4。基于16S rRNA基因的系统发育分析表明，它属于副杆状菌属，与土壤副杆状菌P. soli KCTC 12984亲缘关系最近，相似度为96.03%。菌株4-7-4与土壤副杆状菌P. soli KCTC 12984之间的平均核苷酸同一性和数字DNA-DNA杂交率分别为78.6%和21.6%，均低于划分物种的阈值，支持其为副杆状菌属的一个新物种。主要极性脂包括磷脂酰乙醇胺、未鉴定的氨基脂和四种未鉴定的脂类。主要呼吸醌为MK-7。主要细胞脂肪酸为总和特征3（C ω7c和/或C ω6c）和异-C。菌株4-7-4的基因组为4890729 bp，基因组DNA G+C含量为49.8%。综上所述，菌株4-7-4被鉴定为副杆状菌属的一个新物种，为此提出新名称黄副杆状菌Parapedobacter flavus sp. nov.。模式菌株为4-7-4（=CGMCC1.10938 = KCTC 92351）。
