Parapedobacter flavus sp. nov., isolated from padding of fermentation bed.

A Gram-stain-negative, aerobic, rod-shaped bacterium strain, designated as 4-7-4, was isolated from the padding of a fermentation bed. Phylogenetic analyses based on 16S rRNA gene indicated that it belonged to the genus Parapedobacter and exhibited the closest relationship to P. soli KCTC 12984, with a similarity of 96.03%. The average nucleotide identity and digital DNA-DNA hybridization between strain 4-7-4 and P. soli KCTC 12984 were 78.6% and 21.6% respectively, both of which were below the thresholds for delineating species, supporting it being a novel species of Parapedobacter. The major polar lipids included phosphatidylethanolamine, unidentified aminolipid and four unidentified lipids. The predominant respiratory quinone was MK-7. The major cellular fatty acids were summed feature 3 (C ω7c and/or C ω6c) and iso-C. The genome of strain 4-7-4 was 4890729 bp, and the genomic DNA G + C content was 49.8%. In summary, strain 4-7-4 was identified as a novel species of Parapedobacter, for which the name Parapedobacter flavus sp. nov. was proposed. The type strain was 4-7-4 (=CGMCC1.10938 = KCTC 92351).