Energy Materials and Process, BK 21, Hanyang University, Seoul 133-791, Republic of Korea.
Microbiological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4000-4005. doi: 10.1099/ijs.0.047100-0. Epub 2013 May 24.
A Gram-staining-negative, catalase- and oxidase-positive, non-motile bacterium, designated strain KOPRI 21160(T), was isolated from Antarctic soil. Based on 16S rRNA gene sequence analysis, strain KOPRI 21160(T) was found to belong to the genus Polaribacter. Sequence similarity between strain KOPRI 21160(T) and the type strains of species of the genus Polaribacter was 94.2-98.3 %. The nearest phylogenetic neighbours of strain KOPRI 21160(T) were Polaribacter butkevichii KCTC 12100(T) (98.3 % similarity) and Polaribacter irgensii KCTC 23136(T) (97.5 %). DNA-DNA relatedness was 50.6 %, between strain KOPRI 21160(T) and P. butkevichii KCTC 12100(T), and 45.2 % between strain KOPRI 21160(T) and P. irgensii KCTC 23136(T). Strain KOPRI 21160(T) grew at 4-37 °C and at pH 7.0-8.5. It could hydrolyse DNA, starch and Tweens 20, 40, 60 and 80. Menaquinone-6 (MK-6) was the only respiratory quinone, and iso-C15 : 0, iso-C15 : 0 3-OH and C15 : 1ω6c were the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and one unidentified lipid. The DNA G+C content was 30.0 mol%. Based on data from our polyphasic study, the organism is considered to represent a novel species of the genus Polaribacter, for which we propose the name Polaribacter sejongensis sp. nov. The type strain is KOPRI 21160(T) ( = KCTC 23670(T) = JCM 18092(T)). Emended descriptions of the genus Polaribacter, Polaribacter butkevichii Nedashkovskaya et al. 2005 and Polaribacter irgensii Gosink et al. 1998 are also proposed.
一株革兰氏阴性、触酶和氧化酶阳性、不运动的细菌，被命名为 KOPRI 21160(T)，从南极土壤中分离得到。基于 16S rRNA 基因序列分析，菌株 KOPRI 21160(T)属于 Polaribacter 属。菌株 KOPRI 21160(T)与该属中种的模式菌株的序列相似性为 94.2-98.3%。与菌株 KOPRI 21160(T)最近的系统发育近邻是 Polaribacter butkevichii KCTC 12100(T)(98.3%相似性)和 Polaribacter irgensii KCTC 23136(T)(97.5%相似性)。菌株 KOPRI 21160(T)与 P. butkevichii KCTC 12100(T)之间的 DNA-DNA 同源性为 50.6%，与 P. irgensii KCTC 23136(T)之间的 DNA-DNA 同源性为 45.2%。菌株 KOPRI 21160(T)在 4-37°C 和 pH 7.0-8.5 的条件下生长。它可以水解 DNA、淀粉和吐温 20、40、60 和 80。menaquinone-6 (MK-6)是唯一的呼吸醌，主要的细胞脂肪酸是 iso-C15:0、iso-C15:0 3-OH 和 C15:1ω6c。主要的极性脂是磷脂酰乙醇胺、两种未鉴定的胺脂和一种未鉴定的脂。DNA G+C 含量为 30.0mol%。基于多相研究的数据，该生物被认为代表了 Polaribacter 属的一个新种，我们建议将其命名为 Polaribacter sejongensis sp. nov.。模式菌株为 KOPRI 21160(T)(=KCTC 23670(T)=JCM 18092(T))。还提出了对 Polaribacter 属、Polaribacter butkevichii Nedashkovskaya 等人的描述性修正。2005 年和 Polaribacter irgensii Gosink 等人。1998 年。
