National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, 236-8648, Japan.
National Fisheries University, Shimonoseki, 759-6595, Japan.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1665-1672. doi: 10.1099/ijs.0.041434-0. Epub 2012 Aug 17.
Three Gram-negative, non-motile, strictly aerobic strains, designated LNM-20(T), LCM-1 and LAM-13, were isolated from thalli of the marine red alga Porphyra yezoensis. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates were associated with the genus Polaribacter in the family Flavobacteriaceae and were most closely related to Polaribacter dokdonensis DSW-5(T) (96.2 % 16S rRNA gene sequence similarity) and Polaribacter gangjinensis K17-16(T) (95.0 %). The DNA G+C content of the isolates was 28.6-29.2 mol%. DNA-DNA hybridization analysis showed that the isolates belonged to a single species distinct from both of their closest relatives. The only isoprenoid quinone detected was menaquinone-6. The main polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The major fatty acids were iso-C15 : 0, iso-C15 : 1ω10c and iso-C15 : 0 3-OH. The phenotypic features of strain LNM-20(T) differed from those of their closest relatives in several regards (colony colour, growth with 1 % NaCl and on TSA plus 2.5 % NaCl, hydrolysis of Tweens 40 and 80, and oxidization of five carbon compounds). On the basis of phylogenetic, chemotaxonomic and phenotypic analysis, the isolates represent a novel species in the genus Polaribacter, for which the name Polaribacter porphyrae sp. nov. is proposed. The type strain is LNM-20(T) ( = LMG 26671(T) = NBRC 108759(T)). Emended descriptions of the genus Polaribacter and P. dokdonensis and P. gangjinensis are also proposed.
三株革兰氏阴性、非运动性、严格需氧的菌株，分别命名为 LNM-20(T)、LCM-1 和 LAM-13，从海洋红藻紫菜的藻体中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，这些分离株与黄杆菌科的 Polaribacter 属有关，与 Polaribacter dokdonensis DSW-5(T)（96.2% 16S rRNA 基因序列相似性）和 Polaribacter gangjinensis K17-16(T)（95.0%）最为密切相关。分离株的 DNA G+C 含量为 28.6-29.2mol%。DNA-DNA 杂交分析表明，这些分离株属于一个与它们最接近的两个亲缘种都不同的单一物种。唯一检测到的异戊烯醌是甲萘醌-6。主要的极性脂是磷脂酰乙醇胺、两种未鉴定的氨基脂和两种未鉴定的脂。主要脂肪酸是 iso-C15：0、iso-C15：1ω10c 和 iso-C15：0 3-OH。与最接近的亲缘种相比，LNM-20(T) 菌株的表型特征在几个方面有所不同（菌落颜色、在 1%NaCl 和 TSA 加 2.5%NaCl 上的生长、分解吐温 40 和 80、氧化五种碳化合物）。基于系统发育、化学分类和表型分析，这些分离株代表了 Polaribacter 属的一个新种，建议将其命名为 Polaribacter porphyrae sp. nov.。模式菌株为 LNM-20(T)（=LMG 26671(T)=NBRC 108759(T)）。还对 Polaribacter 属和 P. dokdonensis 和 P. gangjinensis 的描述进行了修正。
