Pós-Graduação em Sanidade, Segurança Alimentar e Ambiental no Agronegócio, Instituto Biológico de São Paulo (IB), Avenida Conselheiro Rodrigues Alves 1252, Bairro Vila Mariana, 04014-002 São Paulo, SP, Brazil.
Biomed Res Int. 2013;2013:425646. doi: 10.1155/2013/425646. Epub 2013 Apr 28.
Bovine leukemia virus (BLV) was investigated in the central nervous system (CNS) of cattle with neurological syndrome. A total of 269 CNS samples were submitted to nested-PCR (BLV env gene gp51), and the viral genotypes were identified. The nested-PCR was positive in 4.8% (13/269) CNS samples, with 2.7% (2/74) presenting at histological examination lesions of nonpurulent meningoencephalitis (NPME), whereas 5.6% (11/195) not presenting NPME (P > 0.05). No samples presented lymphosarcoma. The PCR products (437 bp) were sequenced and submitted to phylogenetic analysis by neighbor-joining and maximum composite likelihood methods, and genotypes 1, 5, and 6 were detected, corroborating other South American studies. The genotype 6 barely described in Brazil and Argentina was more frequently detected in this study. The identity matrices showed maximum similarity (100%) among some samples of this study and one from Argentina (FJ808582), recovered from GenBank. There was no association among the genotypes and NPME lesions.
本研究调查了具有神经症状的牛的中枢神经系统（CNS）中的牛白血病病毒（BLV）。共提交了 269 份 CNS 样本进行巢式 PCR（BLV env 基因 gp51），并鉴定了病毒基因型。巢式 PCR 在 4.8%（13/269）的 CNS 样本中呈阳性，其中 2.7%（2/74）在组织学检查中出现非化脓性脑膜脑炎（NPME）病变，而 5.6%（11/195）未出现 NPME（P>0.05）。没有样本出现淋巴肉瘤。PCR 产物（437bp）被测序，并通过邻接法和最大简约法进行系统发育分析，检测到基因型 1、5 和 6，与其他南美研究结果一致。在巴西和阿根廷鲜有描述的基因型 6 在本研究中更频繁地被检测到。身份矩阵显示，本研究的一些样本与来自 GenBank 的阿根廷的一个样本（FJ808582）之间具有最大相似性（100%）。基因型与 NPME 病变之间无关联。
