Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Invest Ophthalmol Vis Sci. 2013 Jun 27;54(6):4394-402. doi: 10.1167/iovs.12-11138.
To investigate erythrocyte aggregates in parafoveal capillaries by adaptive optics scanning laser ophthalmoscopy (AO-SLO).
AO-SLO videos were acquired from the parafoveal areas of one eye in 10 healthy subjects. Erythrocyte aggregates were detected as "dark tails" that were darker regions than vessel shadows. The lengths of the dark tails were measured in target capillaries, and their time-dependent changes in length were analyzed using spatiotemporal images. The dark tail elongation rate was calculated as the change of dark tail length per unit length of the target capillary.
The overall average dark tail length was 112.1 ± 36.9 μm. The dark tail became longer in a time-dependent manner in every monitored capillary (P < 0.0001). The dark tail elongation rate and average velocity were 0.51 ± 0.37 and 1.49 ± 0.36 mm/s, respectively.
AO-SLO can be used for noninvasive and direct monitoring of blood dynamics in the retinal microvasculature without dying agents. Erythrocyte aggregates were detected as dark tails and were elongated in a time-dependent manner in the parafoveal capillaries of normal subjects. Monitoring the characteristics of dark tails has promising potential for evaluating retinal hemodynamics.
利用自适应光学扫描激光检眼镜（AO-SLO）研究黄斑区毛细血管中的红细胞聚集。
对 10 名健康受试者的一只眼的黄斑区进行 AO-SLO 视频采集。红细胞聚集被检测为比血管阴影更暗的“暗尾”。在目标毛细血管中测量暗尾的长度，并使用时空图像分析其长度随时间的变化。暗尾伸长率计算为目标毛细血管单位长度的暗尾长度变化。
总平均暗尾长度为 112.1 ± 36.9 μm。每个监测的毛细血管中的暗尾都呈现出时间依赖性的变长趋势（P < 0.0001）。暗尾伸长率和平均速度分别为 0.51 ± 0.37 和 1.49 ± 0.36 mm/s。
AO-SLO 可用于非侵入性和直接监测视网膜微血管内的血液动力学，而无需死亡剂。红细胞聚集被检测为暗尾，并在正常受试者的黄斑区毛细血管中呈现出时间依赖性的伸长。监测暗尾的特征在评估视网膜血液动力学方面具有广阔的应用前景。
