Dr. Punjabrao Medical College, Amravati, India.
Indian J Pharmacol. 2013 Mar-Apr;45(2):184-6. doi: 10.4103/0253-7613.108314.
To determine antimutagenic activity of Cassia auriculata Linn. on chromosomal damage induced by cyclophosphamide (CP).
In the present investigation, four groups of six Swiss albino mice in each group were used. Excepting for the first group all the remaining groups were treated with CP (50 mg/kg). Mice of third and fourth group were treated with ethyl acetate extract of C. auriculata Linn. at 100 mg/kg and 200 mg/kg with CP. Metaphase of bone marrow cells of all animals were analyzed for qualitative and quantitative chromosomal aberrations. Break, fragment, deletion, Polyploidy, pulverized, ring and total aberration were observed.
Flavonoids rich extracts of root of C. auriculata Linn. provided significant protection (P < 0.05) against CP induced chromosomal aberration. Total chromosomal aberration was found to be 12.16 and 7.33% in 100 and 200 mg/kg of extract treated animals respectively.
From the present study it can was observed that ethyl acetate extract of C. auriculata Linn possess significant anti-mutagenic potential against CP induced chromosomal aberration.
研究番泻叶的抗突变活性对环磷酰胺（CP）诱导的染色体损伤的影响。
本研究使用了四组每组六只瑞士白化病小鼠。除第一组外，其余所有组均用 CP（50mg/kg）处理。第三和第四组的小鼠用番泻叶根的乙酸乙酯提取物以 100mg/kg 和 200mg/kg 与 CP 一起处理。所有动物的骨髓细胞中期都进行了定性和定量染色体畸变分析。观察到断裂、片段、缺失、多倍体、粉碎、环和总畸变。
富含类黄酮的番泻叶根提取物对 CP 诱导的染色体畸变提供了显著的保护（P<0.05）。在 100 和 200mg/kg 提取物处理的动物中，总染色体畸变分别为 12.16%和 7.33%。
从本研究中可以观察到，番泻叶的乙酸乙酯提取物对 CP 诱导的染色体畸变具有显著的抗突变潜力。
