Korean Ginseng Center Most Valuable Product and Ginseng Genetic Resource Bank, Kyung Hee University, Yongin 446-701, Korea.
J Ginseng Res. 2011 Sep;35(3):344-51. doi: 10.5142/jgr.2011.35.3.344.
Ginsenoside Rb1is the main component in ginsenosides. It is a protopanaxadiol-type ginsenoside that has a dammarane-type triterpenoid as an aglycone. In this study, ginsenoside Rb1 was transformed into gypenoside XVII, ginsenoside Rd, ginsenoside F2 and compound K by glycosidase from Leuconostoc mesenteroides DC102. The optimum time for the conversion was about 72 h at a constant pH of 6.0 to 8.0 and the optimum temperature was about 30℃. Under optimal conditions, ginsenoside Rb1 was decomposed and converted into compound K by 72 h post-reaction (99%). The enzymatic reaction was analyzed by highperformance liquid chromatography, suggesting the transformation pathway: ginsenoside Rb1→ gypenoside XVII and ginsenoside Rd→ginsenoside F2→compound K.
人参皂苷 Rb1 是人参皂苷中的主要成分。它是一种达玛烷型人参皂苷，其糖苷配基为三萜烯。在本研究中，来源于肠膜明串珠菌 DC102 的糖苷酶将人参皂苷 Rb1 转化为了玉竹新苷 XVII、人参皂苷 Rd、人参皂苷 F2 和化合物 K。在 pH 值为 6.0 到 8.0 恒定、温度约为 30℃的条件下，转化的最佳时间约为 72 小时。在最佳条件下，人参皂苷 Rb1 在反应 72 小时后被分解并转化为化合物 K（99%）。通过高效液相色谱法对酶反应进行分析，表明其转化途径为：人参皂苷 Rb1→玉竹新苷 XVII 和人参皂苷 Rd→人参皂苷 F2→化合物 K。
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