匍枝根霉和新月弯孢霉对人参皂苷Rb1的微生物转化
Microbial transformation of ginsenoside Rb1 by Rhizopus stolonifer and Curvularia lunata.
作者信息
Dong Aling, Ye Min, Guo Hongzhu, Zheng Junhua, Guo Dean
机构信息
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road #38, Beijing 100083, PR China.
出版信息
Biotechnol Lett. 2003 Feb;25(4):339-44. doi: 10.1023/a:1022320824000.
Of 49 microbial strains screened for their capabilities to transform ginsenoside Rb1, Rhizopus stolonifer and Curvularia lunata produced four key metabolites: 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl]-3beta,12beta, 20(S)-trihydroxydammar-24-ene (1), 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl]-3beta,12beta, 20(S)-trihydroxydammar-24-ol (2), 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-3beta, 12beta, 20(S)-trihydroxydammar-24-ene (3), and 3-O-beta-D-glucopyranosyl-3beta, 12beta, 20(S)-trihydroxydammar-24-ene (4), identified by TOF-MS, 1H- and 13C-NMR spectral data. Metabolites 1, 3 and 4 were from the incubation with R. stolonifer, and 1 and 2 from the incubation with C. lunata. Compound 2 was identified as a new compound.
在筛选的49种具有转化人参皂苷Rb1能力的微生物菌株中，匍枝根霉和新月弯孢霉产生了四种关键代谢产物：3-O-[β-D-吡喃葡萄糖基-(1,2)-β-D-吡喃葡萄糖基]-20-O-[β-D-吡喃葡萄糖基]-3β,12β,20(S)-三羟基达玛-24-烯(1)、3-O-[β-D-吡喃葡萄糖基-(1,2)-β-D-吡喃葡萄糖基]-20-O-[β-D-吡喃葡萄糖基]-3β,12β,20(S)-三羟基达玛-24-醇(2)、3-O-[β-D-吡喃葡萄糖基-(1,2)-β-D-吡喃葡萄糖基]-3β,12β,20(S)-三羟基达玛-24-烯(3)和3-O-β-D-吡喃葡萄糖基-3β,12β,20(S)-三羟基达玛-24-烯(4)，通过飞行时间质谱、1H和13C核磁共振光谱数据鉴定。代谢产物1、3和4来自与匍枝根霉的培养，1和2来自与新月弯孢霉的培养。化合物2被鉴定为一种新化合物。
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