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利用新型 SNP 探针快速鉴定人参品种（Panax ginseng Meyer）。

Rapid Identification of Ginseng Cultivars (Panax ginseng Meyer) Using Novel SNP-Based Probes.

机构信息

National Institute of Horticultural & Herbal Science, Rural Development Administration, Eumseong 369-873, Korea.

出版信息

J Ginseng Res. 2011 Nov;35(4):504-13. doi: 10.5142/jgr.2011.35.4.504.

DOI:10.5142/jgr.2011.35.4.504
PMID:23717098
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3659547/
Abstract

In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P. ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP110, and PGP130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as 'ATA', 'GCC', 'GTA', 'GCA', and 'ACC', respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.

摘要

为了开发一种鉴别五种人参品种（Panax ginseng Meyer）的新系统，我们进行了实时聚合酶链反应的单核苷酸多态性（SNP）基因分型检测。我们针对人参 cv. Yunpoong 的 gDNA 文库克隆中的核苷酸取代设计了 SNP 基因分型检测。从这些 SNP 位点中，我们开发了一组改良的 SNP 特异性荧光探针（PGP74、PGP110 和 PGP130）和新的引物对，以区分五种人参品种。五个品种（Chungpoong、Yunpoong、Gopoong、Kumpoong 和 Sunpoong）的 SNP 类型分别被鉴定为 'ATA'、'GCC'、'GTA'、'GCA' 和 'ACC'。本研究首次报道了利用荧光探针鉴定人参品种。使用荧光探针的 SNP 基因分型检测可用于鉴定人参品种和人参种子管理系统，并保证人参种子的纯度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/bc8ecb8c63b4/grosbr-35-504-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/754f76b0435e/grosbr-35-504-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/347127409a93/grosbr-35-504-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/a51ffa16d067/grosbr-35-504-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/6c178f101018/grosbr-35-504-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/87d30e1559ee/grosbr-35-504-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/bc8ecb8c63b4/grosbr-35-504-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/754f76b0435e/grosbr-35-504-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/347127409a93/grosbr-35-504-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/a51ffa16d067/grosbr-35-504-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/6c178f101018/grosbr-35-504-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/87d30e1559ee/grosbr-35-504-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0919/3659547/bc8ecb8c63b4/grosbr-35-504-g006.jpg

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本文引用的文献

1
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Meat Sci. 2003 Sep;65(1):599-607. doi: 10.1016/S0309-1740(02)00253-X.
2
Development of molecular markers for the determination of the new cultivar 'Chunpoong' in Panax ginseng C. A. Meyer associated with a major latex-like protein gene.人参新栽培品种‘春蓬’的分子标记开发与主要乳状蛋白基因相关联。
Biol Pharm Bull. 2010;33(2):183-7. doi: 10.1248/bpb.33.183.
3
亚洲人参和西洋参根的从头转录组组装及基因相关单核苷酸多态性标记的鉴定。
Mol Genet Genomics. 2015 Jun;290(3):1055-65. doi: 10.1007/s00438-014-0974-6. Epub 2014 Dec 20.
4
EST-SSR Marker Sets for Practical Authentication of All Nine Registered Ginseng Cultivars in Korea.EST-SSR 标记可用于鉴定韩国 9 种人参栽培品种。
J Ginseng Res. 2012 Jul;36(3):298-307. doi: 10.5142/jgr.2012.36.3.298.
A PCR-based SNP marker for specific authentication of Korean ginseng (panax ginseng) cultivar "Chunpoong".
基于 PCR 的 SNP 标记用于鉴定高丽参（Panax ginseng）品种“春蓬”的特异性。
Mol Biol Rep. 2010 Feb;37(2):1053-7. doi: 10.1007/s11033-009-9827-5.
4
Molecular identification of the Korean ginseng cultivar "Chunpoong" using the mitochondrial nad7 intron 4 region.利用线粒体nad7内含子4区域对高丽参品种“春坪”进行分子鉴定。
Mitochondrial DNA. 2009 Apr-Jun;20(2-3):41-5. doi: 10.1080/19401730902856738.
5
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Biochim Biophys Acta. 2005 Jun 10;1740(3):319-25. doi: 10.1016/j.bbadis.2004.10.010. Epub 2004 Nov 5.
6
Reciprocal activity of ginsenosides in the production of proinflammatory repertoire, and their potential roles in neuroprotection in vivo.人参皂苷在促炎细胞因子产生中的相互作用及其在体内神经保护中的潜在作用。
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7
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J Agric Food Chem. 2005 Jun 1;53(11):4554-60. doi: 10.1021/jf0500841.
8
Assessment of two flexible and compatible SNP genotyping platforms: TaqMan SNP Genotyping Assays and the SNPlex Genotyping System.两种灵活且兼容的单核苷酸多态性（SNP）基因分型平台的评估：TaqMan SNP基因分型分析和SNPlex基因分型系统。
Mutat Res. 2005 Jun 3;573(1-2):111-35. doi: 10.1016/j.mrfmmm.2005.01.008.
9
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Acta Pharmacol Sin. 2005 Feb;26(2):143-9. doi: 10.1111/j.1745-7254.2005.00034.x.
10
Effects of Panax ginseng extract on lipid metabolism in humans.人参提取物对人体脂质代谢的影响。
Pharmacol Res. 2003 Nov;48(5):511-3. doi: 10.1016/s1043-6618(03)00189-0.