Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Biosens Bioelectron. 2013 Nov 15;49:79-85. doi: 10.1016/j.bios.2013.05.003. Epub 2013 May 9.
We developed a new electrochemiluminescence (ECL) platform for ultrasensitive and selective detection of leukemia cells. In order to construct the platform, the nonporous gold with controllable three-dimensional porosity and good conductivity was used to modify the screen-printed carbon electrode. The carbon quantum dots (CQDs) coated ZnO nanosphere (ZnO@CQDs) were used as good ECL label with low cytotoxicity and good biocompatibility. Structure characterization was obtained by means of transmission electron microscopy and scanning electron microscopy images. The aptamer was used for cell capture and the concanavalin A conjugated ZnO@CQDs was used for selective recognition of the cell surface carbohydrate. The proposed method showed a good analytical performance for the detection of K562 cells ranging from 1.0 × 10(2) to 2.0 × 10(7) cells mL(-1) with a detection limit of 46 cells mL(-1). The as-proposed device has the advantages of high sensitivity, nice specificity and good stability and could offer great promise for sensitive detection of leukemia cells in response to therapy.
我们开发了一种新的用于超灵敏和选择性检测白血病细胞的电化学发光（ECL）平台。为了构建该平台，使用具有可控三维多孔性和良好导电性的无孔金来修饰丝网印刷碳电极。涂覆有 ZnO 纳米球（ZnO@CQDs）的碳量子点（CQDs）作为具有低细胞毒性和良好生物相容性的良好 ECL 标记物。通过透射电子显微镜和扫描电子显微镜图像获得结构特征。适体用于细胞捕获，而 ConA 缀合的 ZnO@CQDs 用于选择性识别细胞表面碳水化合物。所提出的方法对 K562 细胞的检测表现出良好的分析性能，检测范围为 1.0×10(2)到 2.0×10(7)细胞 mL(-1)，检测限为 46 个细胞 mL(-1)。该设备具有灵敏度高、特异性好、稳定性好等优点，有望用于治疗后对白血病细胞的灵敏检测。
