Schools of Anatomy, Physiology & Human Biology, The University of Western Australia, Perth, Western Australia 6009, Australia.
Women's & Infants' Health, and The University of Western Australia, Perth, Western Australia 6009, Australia.
J Lipid Res. 2013 Aug;54(8):2247-2254. doi: 10.1194/jlr.M039842. Epub 2013 May 30.
Placental inflammation is associated with several pregnancy disorders. Inflammation is limited by anti-inflammatory and proresolving mechanisms, the latter partly mediated by resolvins and protectins derived from omega-3 polyunsaturated fatty acids (n-3PUFA). We examined effects of dietary n-3PUFAs on levels of resolvins, protectins, and lipoxygenase (ALOX) enzymes in the rat placenta. Rats consumed standard (Std) or high n-3PUFA (Hn3) diets from day 1 of pregnancy; tissues were collected on day 17 or 22 (term = day 23). Maternal Hn3 diet increased resolvin and protectin precursors, 18R/S-HEPE (P < 0.001), and 17R/S-HDHA (P < 0.01) at both days. Resolvins (17R-RvD1 and RvD1) increased at day 22 (P < 0.001) after Hn3 consumption, coincident with higher Alox15b and Alox5 mRNA expression, while RvD2 increased at both days (P < 0.05). Protectins, PD1, and 10S,17S-DiHDHA increased over late gestation (P < 0.001), coincident with higher Alox15 mRNA expression (P < 0.001) and further increased with Hn3 diet (P < 0.05). Maternal systemic and placental proinflammatory mediators were not suppressed by Hn3 diet; systemic IL1β, placental Il1β, and Il6 mRNA expression increased marginally with Hn3 at day 22 (P < 0.001), while Ptgs1 (Cox1) expression increased both days (P < 0.05). Our data indicate that maternal n-3PUFA supplementation enhances expression of enzymes in the n-3PUFA metabolic pathway and increases placental levels of resolvins and protectins.
胎盘炎症与多种妊娠疾病有关。炎症受到抗炎和促解决机制的限制，后者部分由源自ω-3 多不饱和脂肪酸（n-3PUFA）的 resolvins 和 protectins 介导。我们研究了饮食中 n-3PUFA 对大鼠胎盘内 resolvins、protectins 和脂氧合酶（ALOX）酶水平的影响。大鼠从妊娠第 1 天开始摄入标准（Std）或高 n-3PUFA（Hn3）饮食；在第 17 天或 22 天（足月=第 23 天）收集组织。母体 Hn3 饮食增加了 18R/S-HEPE（P < 0.001）和 17R/S-HDHA（P < 0.01）在这两天的前体物质。在 Hn3 消费后第 22 天（P < 0.001）， resolvins（17R-RvD1 和 RvD1）增加，同时 Alox15b 和 Alox5 mRNA 表达增加，而 RvD2 在这两天均增加（P < 0.05）。在妊娠后期， protectins、PD1 和 10S,17S-DiHDHA 增加（P < 0.001），同时 Alox15 mRNA 表达增加（P < 0.001），并随着 Hn3 饮食进一步增加（P < 0.05）。母体系统和胎盘促炎介质未被 Hn3 饮食抑制；母体系统 IL1β、胎盘 Il1β 和 Il6 mRNA 表达在 Hn3 第 22 天略有增加（P < 0.001），而 Ptgs1（Cox1）表达在这两天均增加（P < 0.05）。我们的数据表明，母体 n-3PUFA 补充增强了 n-3PUFA 代谢途径中酶的表达，并增加了胎盘内 resolvins 和 protectins 的水平。
