Vojdani Aristo
Immunosciences Laboratory, Inc., 822 S. Robertson Boulevard, Suite 312, Los Angeles, CA 90035, USA.
ISRN Allergy. 2011 Oct 27;2011:950104. doi: 10.5402/2011/950104. Print 2011.
Intestinal T cells from gluten sensitivity/celiac disease patients respond to a heterogeneous array of peptides. Our study extended this heterogeneity to humoral immune response to various wheat proteins and peptides in patients with gluten sensitivity or Crohn's disease. IgG and IgA antibodies in sera from those patients and healthy control subjects were measured against an array of wheat antigens and peptides. In gluten-sensitive patients, IgG reacted most against transglutaminase, prodynorphin, wheat extract, and α-, γ-, and ω-gliadin; IgA reacted most against wheat then transglutaminase, glutenin, and other peptides. In the sera of Crohn's disease patients, IgG reacted most against wheat and wheat germ agglutinin then transglutaminase, prodynorphin, α-, and γ-gliadin; IgA reacted foremost against prodynorphin then transglutaminase and α-gliadin. These results showed a substantial heterogeneity in the magnitude of IgG and IgA response against various wheat antigens and peptides. Measurements of IgG and IgA antibodies against such an array of wheat peptides and antigens can enhance the sensitivity and specificity of serological assays for gluten sensitivity and celiac disease and may also detect silent celiac disease or its overlap with inflammatory bowel disease.
麸质敏感/乳糜泻患者的肠道T细胞对多种不同的肽产生反应。我们的研究将这种异质性扩展到麸质敏感或克罗恩病患者对各种小麦蛋白和肽的体液免疫反应。检测了这些患者和健康对照者血清中针对一系列小麦抗原和肽的IgG和IgA抗体。在麸质敏感患者中，IgG对转谷氨酰胺酶、前强啡肽、小麦提取物以及α-、γ-和ω-麦醇溶蛋白反应最强；IgA对小麦、然后是转谷氨酰胺酶、麦谷蛋白和其他肽反应最强。在克罗恩病患者的血清中，IgG对小麦和小麦胚芽凝集素反应最强，然后是转谷氨酰胺酶、前强啡肽、α-和γ-麦醇溶蛋白；IgA首先对前强啡肽反应，然后是转谷氨酰胺酶和α-麦醇溶蛋白。这些结果表明，针对各种小麦抗原和肽的IgG和IgA反应强度存在显著异质性。检测针对此类小麦肽和抗原的IgG和IgA抗体可提高麸质敏感和乳糜泻血清学检测的敏感性和特异性，还可能检测出无症状乳糜泻或其与炎症性肠病的重叠情况。
