Immunosciences Lab., Inc., 822 S. Robertson Blvd., Ste. 312, Los Angeles, CA 90035, USA.
Department of Clinical Sciences, Bastyr University California, 4106 Sorrento Valley Blvd, San Diego, CA 92121, USA.
Nutrients. 2013 Dec 19;6(1):15-36. doi: 10.3390/nu6010015.
The aim of this study was to look for the presence of IgG, IgM, and IgA antibodies against two widely consumed foods, wheat and milk, in a relatively large number of specimens. As wheat, milk, and their antigens have been found to be involved in neuroimmune disorders, we measured the co-occurrence of their antibodies against various neural antigens. We assessed the reactivity of sera from 400 donors to wheat and milk proteins, GAD-65, cerebellar, MBP, and MOG. Statistical analysis showed significant clustering when certain wheat and milk protein antibodies were cross-referenced with neural antibodies. Approximately half of the sera with antibody elevation against gliadin reacted significantly with GAD-65 and cerebellar peptides; about half of the sera with elevated antibodies against α + β-casein and milk butyrophilin also showed antibody elevation against MBP and MOG. Inhibition studies showed that only two out of four of the samples with elevated cerebellar or MOG antibodies could be inhibited by gliadin or α + β-casein, confirming individual variation in epitope recognition. We conclude that a subgroup of blood donors, due to a breakdown in immunological tolerance, may react and produce significant levels of antibodies (p-values less than 0.05) against wheat and milk antigens that cross-react with different neural antigens, which may have broader implications in the induction of neuroimmune reactivities.
本研究旨在寻找大量样本中针对两种广泛食用的食物（小麦和牛奶）的 IgG、IgM 和 IgA 抗体的存在。由于小麦、牛奶及其抗原已被发现与神经免疫紊乱有关，我们测量了它们针对各种神经抗原的抗体的共同出现情况。我们评估了 400 名供体对小麦和牛奶蛋白、GAD-65、小脑、MBP 和 MOG 的反应性。统计分析显示，当某些小麦和牛奶蛋白抗体与神经抗体交叉参考时，存在显著聚类。大约一半对麦醇溶蛋白抗体升高的血清与 GAD-65 和小脑肽显著反应；约一半对 α+β-酪蛋白和牛奶乳铁蛋白抗体升高的血清也对 MBP 和 MOG 抗体升高。抑制研究表明，只有四个升高的小脑或 MOG 抗体样本中的两个可以被麦醇溶蛋白或 α+β-酪蛋白抑制，这证实了表位识别的个体差异。我们得出结论，由于免疫耐受的破坏，一小部分献血者可能会对与不同神经抗原发生交叉反应的小麦和牛奶抗原产生反应并产生显著水平的抗体（p 值小于 0.05），这可能对诱导神经免疫反应具有更广泛的影响。
