[大蓟、魁蓟和线叶蓟中蒙花苷和香叶木素的含量比较]

[Content comparison of buddleoside and pectolinarin in Cirsium japonicum, C. leo and C. leducei].

作者信息

Li Zhi-Hao, Song Liang-Ke, Wang Xiao-Ning, Wang Yan, Wu Shu-Xing, Yu Cheng-Long, Zong Yu-Ying

机构信息

Life Science and Engineering College of Southwest Jiaotong University, Chengdu 610031, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2013 Mar;38(5):674-7.

PMID:23724673

Abstract

OBJECTIVE

To compare Cirsium japonicum characteristics with C. leo and C. leducei, along with the content of buddleoside and pectolinarin, and lay the foundation for the quality control of C. japonicum.

METHOD

Samples were collected and the relevant drugs were bought. The samples were divided into root, stem, leaf and flower, and the content of buddleoside and pectolinarin was determine by the HPLC. Chromatographic column: Waters XBridge C18 (4.6 mm x 250 mm), mobile phase: methanol-water (45: 55), measurement wavelength: 326 nm, flow rate: 0.8 mL x min(-1), column temperature: 30 degrees C. RESULT AND CONDUSION: Standard curve equation of buddleoside: Y = 74 064X-47 748, R2 = 0.991. Standard curve equation of pectolinarin: Y = 1 711 64X - 180 707, R2 = 0.999. The content of buddleoside: C. japonicum leaf was 1.987 3%, C. leo leaf 1.412 2%, C. leducei leaf 0.149 2%. The content of buddleoside was lower in root and stem. Pectolinarin was not detected in the C. japonicum and C. leo. The pectolinarin content was 0.069 0% in C. leducei leaf.

摘要

目的

比较日本蓟与大蓟、线叶蓟的特征以及蒙花苷和橙皮苷的含量，为日本蓟的质量控制奠定基础。

方法

采集样品并购买相关药材。将样品分为根、茎、叶和花，采用高效液相色谱法测定蒙花苷和橙皮苷的含量。色谱柱：Waters XBridge C18（4.6 mm×250 mm），流动相：甲醇-水（45∶55），检测波长：326 nm，流速：0.8 mL·min⁻¹，柱温：30℃。结果与结论：蒙花苷标准曲线方程：Y = 74 064X - 47 748，R² = 0.991。橙皮苷标准曲线方程：Y = 1 711 64X - 180 707，R² = 0.999。蒙花苷含量：日本蓟叶为1.987 3%，大蓟叶为1.412 2%，线叶蓟叶为0.149 2%。根和茎中蒙花苷含量较低。日本蓟和大蓟中未检测到橙皮苷。线叶蓟叶中橙皮苷含量为0.069 0%。