Cao Chao, Sun Shi-Fang, Lv Dan, Chen Zhong-Bo, Ding Qun-Li, Deng Zai-Chun
Department of Respiratory Medicine, the Affiliated Hospital of School of Medicine, Ningbo University, Ningbo, China.
Asian Pac J Cancer Prev. 2013;14(4):2443-6. doi: 10.7314/apjcp.2013.14.4.2443.
Published data have shown that the levels of vascular endothelial growth factor (VEGF) and soluble VEGF receptor-1 (sVEGFR-1) in plasma and pleural effusion might be usefulness for lung cancer diagnosis. Here, we performed a prospective study to investigate the utility of VEGF and sVEGFR-1 in bronchoalveolar lavage fluid (BALF) for differential diagnosis of primary lung cancer. A total of 56 patients with solitary pulmonary massed by chest radiograph or CT screening were enrolled in this study. BALF and plasma samples were obtained from all patients and analyzed for VEGF and sVEGFR-1 using a commercially available sandwich ELISA kit. The results showed that the levels of VEGF in BALF were significantly higher in patients with a malignant pulmonary mass compared with patients with a benign mass (P < 0.001). However, no significant difference of sVEGFR-1 in BALF was found between malignant and non-malignant groups (P = 0.43). With a cut-off value of 214 pg/ml, VEGF showed a sensitivity and specificity of 81.8% and 84.2%, respectively, in predicting the malignant nature of a solitary pulmonary mass. Our study suggests that VEGF is significantly increased in BALF among patients with lung cancer than in benign diseases. Measurement of VEGF in BALF might be helpful for differential diagnosis of primary lung cancer.
已发表的数据表明，血浆和胸腔积液中血管内皮生长因子（VEGF）和可溶性VEGF受体-1（sVEGFR-1）水平可能有助于肺癌诊断。在此，我们进行了一项前瞻性研究，以探讨支气管肺泡灌洗液（BALF）中VEGF和sVEGFR-1在原发性肺癌鉴别诊断中的作用。本研究共纳入56例经胸部X线或CT筛查发现孤立性肺肿块的患者。采集所有患者的BALF和血浆样本，使用市售夹心ELISA试剂盒分析VEGF和sVEGFR-1。结果显示，与良性肿块患者相比，恶性肺肿块患者BALF中的VEGF水平显著更高（P < 0.001）。然而，恶性组和非恶性组BALF中sVEGFR-1无显著差异（P = 0.43）。以214 pg/ml为临界值，VEGF在预测孤立性肺肿块的恶性性质时，敏感性和特异性分别为81.8%和84.2%。我们的研究表明，肺癌患者BALF中的VEGF比良性疾病患者显著升高。检测BALF中的VEGF可能有助于原发性肺癌的鉴别诊断。
