Kelleher D, Feighery C, Weir D G
Department of Clinical Medicine, Trinity College, Dublin Medical School.
Digestion. 1990;45(3):158-65. doi: 10.1159/000200238.
Chemiluminescence (CL) is a simple quantitative assay of polymorphonuclear leucocyte (PMNL) oxidative metabolism. PMNL CL was found to be significantly higher in patients with chronic inflammatory bowel disease than in normal controls (167 +/- 60 vs. 139 +/- 50 mV/10(5) cells, p less than 0.05). There were no significant differences between patients with ulcerative colitis and Crohn's disease. Disease controls with rheumatoid arthritis and with bronchiectasis also demonstrated elevated CL. These results were obtained using a two-step gelatin/Ficoll-Hypaque procedure for PMNL separation. However when PMNLs were prepared using a one-step Ficoll-Hypaque procedure PMNL CL was found to be depressed in chronic inflammatory bowel disease (CIBD). It was demonstrated that this disparity was caused by the elimination of low-density neutrophils with high CL production by the one-step procedure. These data indicate that reports of abnormal in vitro neutrophil function in CIBD should be interpreted with caution since separation techniques which are satisfactory in normal individuals may significantly influence results in patients with inflammatory diseases. Furthermore these data indicate the presence of a subpopulation of activated low-density PMNL in patients with CIBD.
化学发光（CL）是一种检测多形核白细胞（PMNL）氧化代谢的简单定量分析方法。研究发现，慢性炎症性肠病患者的PMNL CL显著高于正常对照组（167±60对139±50 mV/10⁵细胞，p<0.05）。溃疡性结肠炎患者和克罗恩病患者之间无显著差异。类风湿性关节炎和支气管扩张症的疾病对照组也显示CL升高。这些结果是使用两步明胶/聚蔗糖-泛影葡胺法分离PMNL获得的。然而，当使用一步聚蔗糖-泛影葡胺法制备PMNL时，发现慢性炎症性肠病（CIBD）患者的PMNL CL降低。结果表明，这种差异是由于一步法去除了具有高CL产生能力的低密度中性粒细胞所致。这些数据表明，CIBD患者体外中性粒细胞功能异常的报告应谨慎解读，因为在正常个体中令人满意的分离技术可能会显著影响炎症性疾病患者的结果。此外，这些数据表明CIBD患者中存在一群活化的低密度PMNL。
