Nandi S P, Rahman M Z, Momtaz S, Sultana M, Hossain M A
Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.
Transbound Emerg Dis. 2015 Jun;62(3):328-31. doi: 10.1111/tbed.12113. Epub 2013 Jun 4.
Foot-and-mouth disease (FMD) is endemic in Bangladesh and is predominantly due to FMDV serotype O. In 2012, FMD outbreaks were identified in five different districts of Bangladesh. Of 56 symptomatic cattle epithelial tissue samples, diagnostic PCR assay based on 5'-URT detected 38 FMDV infections. Viral genotyping targeting VP1-encoding region confirmed emergence of two distinct serotypes, A and O with an abundance of serotype A in Chittagong and Gazipur districts and serotype O in Pabna and Faridpur. Only single lineage of both A and O was retrieved from samples of five different regions. Sequencing and phylogenetic analysis of VP1 sequences revealed that serotype O sequences were closely related to the Ind 2001 sublineage of Middle East-South Asia (ME-SA) topotype that was previously circulating in Bangladesh, and serotype A sequences belonging to the genotype VII that was dominant in India during the last decade. The results suggest that extensive cross-border animal movement from neighbouring countries is the most likely source of FMDV serotypes in Bangladesh.
口蹄疫（FMD）在孟加拉国呈地方流行，主要由口蹄疫病毒（FMDV）O型引起。2012年，在孟加拉国的五个不同地区发现了口蹄疫疫情。在56份有症状的牛上皮组织样本中，基于5'-URT的诊断性PCR检测出38份FMDV感染。针对编码VP1区域的病毒基因分型证实出现了两种不同的血清型，A和O，其中吉大港和加济布尔地区血清型A占优势，帕布纳和法里德布尔地区血清型O占优势。从五个不同地区的样本中仅获得了A和O的单一谱系。VP1序列的测序和系统发育分析表明，O型血清型序列与先前在孟加拉国流行的中东-南亚（ME-SA）拓扑型的Ind 2001亚谱系密切相关，A 型血清型序列属于过去十年在印度占主导地位的基因型VII。结果表明，来自邻国的广泛跨境动物流动是孟加拉国FMDV血清型最可能的来源。
