School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Evid Based Complement Alternat Med. 2013;2013:718305. doi: 10.1155/2013/718305. Epub 2013 May 2.
Coronary heart disease (CHD) is one of the highest mortality diseases in the world. Traditional Chinese medicine compound Danshen dripping pills (CDDPs) have currently made a great achievement in treating CHD. However, the therapeutic mechanism of CDDP is often poorly interpreted. In this study, a GC-MS-based metabonomic study was conducted to assess the holistic efficacy of CDDP for myocardial infarction in male Sprague-Dawley rats, which were divided into the control group, the sham group, the model group, the control + CDDP group, and the model + CDDP, with CDDP at a dose of 107 mg/kg·d (equal to 1.8 mL/kg·d). The metabonomic findings demonstrated great differences of metabolic pattern among sham, model, and the model + CDDP in the orthogonal partial least squares discriminant analysis (OPLS-DA) models, which coordinated well with the assessment of plasma biochemistry and histopathological assay. Differentially expressed metabolites suggested that energy metabolism, glycolysis, and lipid metabolism might be disrupted by myocardial infarction. Both the potential metabolic biomarkers and the biochemical histopathological indices were regulated effectively by CDDP.
冠心病（CHD）是世界上死亡率最高的疾病之一。中药复方丹参滴丸（CDDPs）在治疗 CHD 方面已取得重大成就。然而，CDDP 的治疗机制往往解释不清。在这项研究中，我们进行了基于 GC-MS 的代谢组学研究，以评估 CDDP 对雄性 Sprague-Dawley 大鼠心肌梗死的整体疗效，将大鼠分为对照组、假手术组、模型组、对照组+CDDP 组和模型组+CDDP 组，CDDP 剂量为 107mg/kg·d（相当于 1.8mL/kg·d）。代谢组学研究发现，在正交偏最小二乘判别分析（OPLS-DA）模型中，假手术组、模型组和模型+CDDP 组之间的代谢模式存在显著差异，与血浆生化和组织病理学评估结果相协调。差异表达的代谢物表明，心肌梗死可能破坏能量代谢、糖酵解和脂质代谢。CDDP 能有效调节潜在的代谢生物标志物和生化组织病理学指标。
