最初，大肠杆菌组装原环：分裂的初始阶段。
In the beginning, Escherichia coli assembled the proto-ring: an initial phase of division.
机构信息
From the Centro Nacional de Biotecnología (CNB), Consejo Superior de Investigaciones Científicas (CSIC), 28049 Madrid, Spain.
From the Centro Nacional de Biotecnología (CNB), Consejo Superior de Investigaciones Científicas (CSIC), 28049 Madrid, Spain.
出版信息
J Biol Chem. 2013 Jul 19;288(29):20830-20836. doi: 10.1074/jbc.R113.479519. Epub 2013 Jun 5.
Abstract
Cell division in Escherichia coli begins by assembling three proteins, FtsZ, FtsA, and ZipA, to form a proto-ring at midcell. These proteins nucleate an assembly of at least 35 components, the divisome. The structuring of FtsZ to form a ring and the processes that effect constriction have been explained by alternative but not mutually exclusive mechanisms. We discuss how FtsA and ZipA provide anchoring of the cytoplasmic FtsZ to the membrane and how a temporal sequence of alternative protein interactions may operate in the maturation and stability of the proto-ring. How the force needed for constriction is generated and how the proto-ring proteins relate to peptidoglycan synthesis remain as the main challenges for future research.
摘要
大肠杆菌的细胞分裂始于组装三种蛋白质，FtsZ、FtsA 和 ZipA，在细胞中部形成一个原环。这些蛋白质启动了至少 35 种成分的组装，即分裂体。FtsZ 形成环的结构和影响收缩的过程已经通过替代但不相互排斥的机制来解释。我们讨论了 FtsA 和 ZipA 如何将细胞质 FtsZ 锚定在膜上，以及替代蛋白质相互作用的时间序列如何在原环的成熟和稳定性中发挥作用。收缩所需的力是如何产生的，以及原环蛋白与肽聚糖合成的关系仍然是未来研究的主要挑战。
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2
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