Oboh Ganiyu, Olabiyi Ayodeji A, Akinyemi Ayodele J, Ademiluyi Adedayo O
J Basic Clin Physiol Pharmacol. 2014 Feb;25(1):21-34. doi: 10.1515/jbcpp-2013-0002.
Various parts of unripe pawpaw (Carica papaya Linn) fruit have been reportedly used for the management or treatment of diabetes mellitus in folklore medicine. Therefore, the present study sought to investigate the inhibitory effects of the aqueous extract of different parts of unripe pawpaw fruit on key enzymes linked to type 2 diabetes (α-amylase and α-glucosidase) and sodium nitroprusside (SNP)-induced lipid peroxidation in rat pancreas in vitro.
The aqueous extracts of the unripe pawpaw (C. papaya) fruit parts were prepared (1:20 w/v) and the ability of the extracts to inhibit α-amylase, α-glucosidase and SNP-induced lipid peroxidation in rat pancreas in vitro was investigated.
The results revealed that all the extracts inhibited α-amylase (IC50=0.87-1.11 mg/mL), α-glucosidase (IC50=1.76-2.64 mg/mL) and SNP-induced lipid peroxidation (IC50=1.99-2.42 mg/mL) in a dose-dependent manner. However, combination of the flesh, seed and peel in equal amounts had the highest inhibitory effect on α-amylase and α-glucosidase activities.
Strong inhibitory activities of the unripe pawpaw fruit against key enzymes linked to type 2 diabetes and SNP-induced lipid peroxidation in rat pancreas could be part of the mechanism by which unripe pawpaw is used in the management/prevention of diabetes mellitus in folk medicine. However, combining the unripe pawpaw fruit parts in equal amounts exhibited synergistic properties on α-amylase and α-glucosidase inhibitory activities.
据报道，未成熟番木瓜（番木瓜属）果实的不同部位在民间医学中被用于管理或治疗糖尿病。因此，本研究旨在体外研究未成熟番木瓜果实不同部位的水提取物对与2型糖尿病相关的关键酶（α-淀粉酶和α-葡萄糖苷酶）以及硝普钠（SNP）诱导的大鼠胰腺脂质过氧化的抑制作用。
制备未成熟番木瓜果实各部位的水提取物（1:20 w/v），并研究提取物在体外抑制大鼠胰腺中α-淀粉酶、α-葡萄糖苷酶和SNP诱导的脂质过氧化的能力。
结果显示，所有提取物均以剂量依赖性方式抑制α-淀粉酶（IC50 = 0.87 - 1.11 mg/mL）、α-葡萄糖苷酶（IC50 = 1.76 - 2.64 mg/mL）和SNP诱导的脂质过氧化（IC50 = 1.99 - 2.42 mg/mL）。然而，等量的果肉、种子和果皮组合对α-淀粉酶和α-葡萄糖苷酶活性具有最高的抑制作用。
未成熟番木瓜果实对与2型糖尿病相关的关键酶以及SNP诱导的大鼠胰腺脂质过氧化具有强大的抑制活性，这可能是未成熟番木瓜在民间医学中用于管理/预防糖尿病的机制的一部分。然而，等量组合未成熟番木瓜果实各部位对α-淀粉酶和α-葡萄糖苷酶的抑制活性表现出协同特性。
