Department of Biochemistry, Federal University of Technology Akure, Akure, Nigeria.
Department of Biochemistry, Federal University Oye, Oye-Ekiti, Nigeria.
J Food Drug Anal. 2016 Jul;24(3):627-634. doi: 10.1016/j.jfda.2016.03.003. Epub 2016 Apr 11.
Acarbose is an antidiabetic drug which acts by inhibiting α-amylase and α-glucosidase activities but with deleterious side effects. Gallic acid (GA) is a phenolic acid that is widespread in plant foods. We therefore investigated the influence of GA on α-amylase and α-glucosidase inhibitory properties of acarbose (in vitro). Aqueous solutions of acarbose and GA were prepared to a final concentration of 25μM each. Thereafter, mixtures of the samples (50% acarbose + 50% GA; 75% acarbose+25% GA; and 25% acarbose+75% GA) were prepared. The results revealed that the combination of 50% acarbose and 50% GA showed the highest α-glucosidase inhibitory effect, while 75% acarbose+25% GA showed the highest α-amylase inhibitory effect. Furthermore, all the samples caused the inhibition of Fe-induced lipid peroxidation (in vitro) in rat pancreatic tissue homogenate, with the combination of 50% acarbose and 50% GA causing the highest inhibition. All the samples also showed antioxidant properties (reducing property, 2,2'-azino-bis (-3-ethylbenzthiazoline-6-sulphonate [ABTS*] and 1,1-diphenyl-2-picrylhydrazyl [DPPH] free radicals scavenging abilities, and Fe chelating ability). Therefore, combinations of GA with acarbose could be employed as antidiabetic therapy, with a possible reduction of side effects of acarbose; nevertheless, the combination of 50% acarbose and 50% GA seems the best.
阿卡波糖是一种抗糖尿病药物，通过抑制α-淀粉酶和α-葡萄糖苷酶的活性起作用，但具有有害的副作用。没食子酸（GA）是一种广泛存在于植物性食物中的酚酸。因此，我们研究了 GA 对阿卡波糖（体外）抑制α-淀粉酶和α-葡萄糖苷酶活性的影响。将阿卡波糖和 GA 的水溶液制备至终浓度为 25μM。然后，制备样品的混合物（50%阿卡波糖+50%GA；75%阿卡波糖+25%GA；和 25%阿卡波糖+75%GA）。结果表明，50%阿卡波糖和 50%GA 的组合显示出最高的α-葡萄糖苷酶抑制作用，而 75%阿卡波糖+25%GA 显示出最高的α-淀粉酶抑制作用。此外，所有样品均导致大鼠胰腺组织匀浆中 Fe 诱导的脂质过氧化（体外）抑制，其中 50%阿卡波糖和 50%GA 的组合抑制作用最强。所有样品还显示出抗氧化特性（还原能力、2,2'-联氮双（-3-乙基苯并噻唑啉-6-磺酸）[ABTS*]和 1,1-二苯基-2-苦基肼[DPPH]自由基清除能力和 Fe 螯合能力）。因此，GA 与阿卡波糖的组合可用于抗糖尿病治疗，可能减少阿卡波糖的副作用；然而，50%阿卡波糖和 50%GA 的组合似乎是最好的。
