Papac D I, Foltz R L
Center for Human Toxicology, University of Utah, Salt Lake City 84108.
J Anal Toxicol. 1990 May-Jun;14(3):189-90. doi: 10.1093/jat/14.3.189.
A previously reported procedure for quantification of LSD in urine was modified to permit measurement of the drug in plasma. After addition of deuterium-labelled LSD, the plasma is extracted and the extract is treated with trifluoroacetylimidazole to convert the LSD to its N-trifluoroacetyl derivative. The derivatized LSD is analyzed by capillary column gas chromatography/negative ion chemical ionization. Plasma fortified with known concentrations of LSD gave linear responses from 0.1 to 3.0 ng/mL with this assay. The method was used to determine pharmacokinetic parameters for LSD after oral administration (1 microgram/kg) to a male volunteer. The apparent plasma half-life was determined to be 5.1 h. The peak plasma concentration of 1.9 ng/mL occurred 3 h after administration.
先前报道的尿液中麦角酸二乙酰胺（LSD）定量方法经过修改，以实现血浆中该药物的测定。加入氘标记的LSD后，提取血浆，提取物用三氟乙酰咪唑处理，将LSD转化为其N-三氟乙酰衍生物。衍生化的LSD通过毛细管柱气相色谱/负离子化学电离进行分析。该测定法对添加已知浓度LSD的血浆给出了0.1至3.0 ng/mL的线性响应。该方法用于测定一名男性志愿者口服给药（1微克/千克）后LSD的药代动力学参数。表观血浆半衰期测定为5.1小时。给药后3小时出现了1.9 ng/mL的血浆峰浓度。
