Laboratory of Retroviruses, Division of Viral Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD 20892, USA.
Viruses. 2013 Jun 6;5(6):1414-30. doi: 10.3390/v5061414.
We have investigated the influence of naturally occurring simian foamy viruses (SFVs) on simian immunodeficiency virus (SIV) infection and disease in Indian rhesus macaques. Animals were divided into two groups based upon presence or absence of SFV; in each group, eight monkeys were injected with SIV(mac239) virus obtained from a molecular clone and four were injected with medium. Blood was collected every two weeks for evaluation of SIV infection based upon T cell-subsets, plasma viral load, development and persistence of virus-specific antibodies, and clinical changes by physical examination and hematology. Comparative analysis of SFV+/SIV+ and SFV-/SIV+ monkey groups indicated statistically significant differences in the plasma viral load between 6-28 weeks, particularly after reaching plateau at 20-28 weeks, in the CD4+ and CD8+ T-cell numbers over the entire study period (2-43 weeks), and in the survival rates evaluated at 49 weeks. There was an increase in the plasma viral load, a decreasing trend in the CD4+ T cells, and a greater number of animal deaths in the SFV+/SIV+ group. The results, although based upon a small number of animals, indicated that pre-existing SFV infection can influence SIV infection and disease outcome in the rhesus macaque model. The study highlights consideration of the SFV status in evaluating results from SIV pathogenesis and vaccine challenge studies in monkeys and indicates the potential use of the SFV/SIV monkey model to study the dynamics of SFV and HIV-1 dual infections, recently reported in humans.
我们研究了天然猴泡沫病毒（SFV）对印度猕猴感染猴免疫缺陷病毒（SIV）和疾病的影响。动物根据是否存在 SFV 分为两组；每组八只猴子注射来源于分子克隆的 SIV（mac239）病毒，四只猴子注射培养基。每两周采集一次血液，通过 T 细胞亚群、血浆病毒载量、病毒特异性抗体的产生和持续存在以及体检和血液学检查的临床变化来评估 SIV 感染情况。SFV+/SIV+和 SFV-/SIV+猴组的比较分析表明，在 6-28 周之间的血浆病毒载量存在统计学显著差异，特别是在 20-28 周达到平台期后，在整个研究期间（2-43 周）的 CD4+和 CD8+T 细胞数量以及在 49 周评估的存活率方面存在统计学显著差异。SFV+/SIV+组的血浆病毒载量增加，CD4+T 细胞呈下降趋势，动物死亡数量更多。尽管基于少数动物，但结果表明，先前存在的 SFV 感染可以影响猕猴模型中的 SIV 感染和疾病结局。该研究强调了在评估猴子中 SIV 发病机制和疫苗挑战研究结果时要考虑 SFV 状态，并表明可以利用 SFV/SIV 猴模型来研究 SFV 和 HIV-1 双重感染的动态，最近在人类中有所报道。
