Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, China.
Appl Microbiol Biotechnol. 2013 Aug;97(16):7141-50. doi: 10.1007/s00253-013-5000-0. Epub 2013 Jun 7.
The bacterium Bacillus amyloliquefaciens anti-CA isolated from mangrove system was found to be able to actively kill Candida albicans isolated from clinic. The bacterial strain anti-CA could produce high level of bioactive substance, amylase and protease in the cheap medium containing 2.0 % soybean meal, 2.0 % wheat flour, pH 6.5 within 26 h. After purification, the main bioactive substance was confirmed to be a cyclic lipopeptide containing a heptapeptide, L-Asp→L-Leu→L-Leu→L-Val→L-Val→L-Glu→L-Leu and a 3-OH fatty acid (15 carbons). In addition to C. albicans, the purified lipopeptide can also kill many yeast strains including Metschnikowia bicuspidata, Candida tropicalis, Yarrowia lipolytica and Saccharomyces cerevisiae. After treated by the purified lipopeptide, both the whole cells and protoplasts of C. albicans were destroyed.
从红树林系统中分离到的解淀粉芽孢杆菌 Bacillus amyloliquefaciens anti-CA 被发现能够主动杀死从临床分离到的白色念珠菌。该菌株 anti-CA 能够在含有 2.0%豆粕、2.0%小麦粉、pH 6.5 的廉价培养基中在 26 小时内产生高水平的生物活性物质、淀粉酶和蛋白酶。经纯化后，主要生物活性物质被确认为一种含有七肽的环状脂肽，序列为 L-Asp→L-Leu→L-Leu→L-Val→L-Val→L-Glu→L-Leu 和一个 3-OH 脂肪酸（15 个碳）。除了白色念珠菌外，该纯化的脂肽还可以杀死许多酵母菌株，包括双叉孢滑霉 Metschnikowia bicuspidata、热带假丝酵母 Candida tropicalis、解脂耶氏酵母 Yarrowia lipolytica 和酿酒酵母 Saccharomyces cerevisiae。经纯化的脂肽处理后，白色念珠菌的整个细胞和原生质体都被破坏了。
