College of Life Sciences, Qingdao University, Qingdao 266071, China.
CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Mar Drugs. 2019 Mar 29;17(4):199. doi: 10.3390/md17040199.
This study was initiated to screen for marine bacterial agents to biocontrol , a serious fungal pathogen of cereal crops. A bacterial strain, isolated from the cold seep in deep sea, exhibited strong growth inhibition against , and the strain was identified and designated as sp. CS30. The corresponding antifungal agents were purified by acidic precipitation, sequential methanol extraction, Sephadex LH-20 chromatography, and reversed phase high-performance liquid chromatography (RP-HPLC), and two antifungal peaks were obtained at the final purification step. After analysis by mass spectrometry (MS) and tandem MS, two purified antifungal agents were deduced to belong to the surfactin family, and designated as surfactin CS30-1 and surfactin CS30-2. Further investigation showed that although the antifungal activity of surfactin CS30-1 is higher than that of surfactin CS30-2, both of them induced the increased generation of reactive oxygen species (ROS) and caused serious damage to the cell wall and cytoplasm, thus leading to the cell death of . Our results also show the differences of the antifungal activity and antifungal mechanism of the different surfactin homologs surfactin CS30-1 and surfactin CS30-2, and highlight them as potential promising agents to biocontrol plant diseases caused by .
本研究旨在筛选海洋细菌剂来防治一种严重的谷类作物真菌病原体。从深海冷泉中分离到的一株细菌对表现出强烈的生长抑制作用，该菌株被鉴定并命名为 sp. CS30。相应的抑菌剂通过酸性沉淀、甲醇连续提取、Sephadex LH-20 层析和反相高效液相色谱（RP-HPLC）进行纯化，在最后一步的纯化中得到了两个抑菌峰。经质谱（MS）和串联 MS 分析，两种纯化的抑菌剂被推断属于表面活性剂家族，并分别命名为表面活性剂 CS30-1 和表面活性剂 CS30-2。进一步研究表明，尽管表面活性剂 CS30-1 的抑菌活性高于表面活性剂 CS30-2，但它们都诱导了活性氧（ROS）的产生增加，并对细胞壁和细胞质造成严重损伤，从而导致 的死亡。我们的结果还显示了不同表面活性剂同系物表面活性剂 CS30-1 和表面活性剂 CS30-2 的抑菌活性和抑菌机制的差异，并强调它们是防治由 引起的植物病害的潜在有前途的药剂。
