Nowak C
Freie Universität Berlin, Institut für Genetik, Germany.
Mutat Res. 1990 Jun;230(2):227-34. doi: 10.1016/0027-5107(90)90060-h.
In this study V79 hamster cells were treated with hypertonic NaCl solutions. A pulse treatment of 30 min made it possible to use hypertonic solutions of up to 1500 mM. Hypertonic treatment induced high frequencies of chromosomal aberrations and the aberration pattern led to the conclusion that hypertonicity acts similarly to S-phase-independent mutagens. We also tested the influence of several parameters on aberration induction and tried to standardize the experimental protocol. The mechanisms involved in aberration production after hypertonic treatment are unknown, we discuss a change in chromatin structure, inhibition of repair processes or protein damage responsible for chromosomal aberrations.
在本研究中，V79仓鼠细胞用高渗NaCl溶液处理。30分钟的脉冲处理使得能够使用高达1500 mM的高渗溶液。高渗处理诱导了高频染色体畸变，畸变模式表明高渗作用类似于不依赖S期的诱变剂。我们还测试了几个参数对畸变诱导的影响，并试图使实验方案标准化。高渗处理后产生畸变所涉及的机制尚不清楚，我们讨论了染色质结构的变化、修复过程的抑制或导致染色体畸变的蛋白质损伤。
