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[凝集试验在医学微生物学中快速鉴定分离出的链球菌的价值]

[Value of agglutination tests for the rapid identification of isolated streptococci in medical microbiology].

作者信息

Malbrunot C, Devine C, Chailloux J, Garrigues M L, Piquée M L, Wargnier A, Bouvet A

机构信息

Laboratoire de Microbiologie, Hôpital Ambroise-Paré, Faculté de Médecine Paris-Ouest, Boulogne, France.

出版信息

Pathol Biol (Paris). 1990 Jun;38(6):665-8.

PMID:2374701
Abstract

The clinical need for rapidly and correctly differentiating pathogenic streptococci into Lancefield groups prompted the development of rapid agglutination techniques by the direct colony method. Pastorex Streptogroupe (Diagnostics Pasteur, France) and Streptex (Wellcome Diagnostics, USA) were tested on 90 streptococcal isolates and compared with the identification obtained by conventional procedures. No cross-reactions were observed with any of the 33 strains of enterococci and of the seven strains representative of other genus among Gram-positive bacteria. When combined with colonial morphology and hemolytic reaction, both Pastorex and Streptex were specific tests for identifying beta-hemolytic streptococci, with Pastorex being slightly faster.

摘要

临床上需要快速、准确地将致病性链球菌区分到兰斯菲尔德菌群中,这促使了通过直接菌落法开发快速凝集技术。对90株链球菌分离株进行了法国巴斯德诊断公司的Pastorex Streptogroupe和美国威康诊断公司的Streptex检测,并与通过传统方法获得的鉴定结果进行了比较。在33株肠球菌和7株革兰氏阳性菌中代表其他属的菌株中均未观察到交叉反应。当与菌落形态和溶血反应相结合时,Pastorex和Streptex都是鉴定β溶血性链球菌的特异性试验,其中Pastorex稍快一些。

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